Publikation: The synthesis of 2′-methylseleno adenosine and guanosine 5′-triphosphates
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2012
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Bioorganic & Medicinal Chemistry. 2012, 20(7), pp. 2416-2418. ISSN 0968-0896. eISSN 1464-3391. Available under: doi: 10.1016/j.bmc.2012.01.044
Zusammenfassung
Modified nucleoside triphosphates (NTPs) represent powerful building blocks to generate nucleic acids with novel properties by enzymatic synthesis. We have recently demonstrated the access to 20-SeCH3-uridine and 20-SeCH3-cytidine derivatized RNAs for applications in RNA crystallography, using the corresponding
nucleoside triphosphates and distinct mutants of T7 RNA polymerase. In the present note, we introduce the chemical synthesis of the novel 20-methylseleno-20-deoxyadenosine and -guanosine 50-triphosphates (20-SeCH3-ATP and 20-SeCH3-GTP) that represent further candidates for the enzymatic RNA synthesis with engineered RNA polymerases.
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540 Chemie
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SANTNER, Tobias, Vanessa SIEGMUND, Andreas MARX, Ronald MICURA, 2012. The synthesis of 2′-methylseleno adenosine and guanosine 5′-triphosphates. In: Bioorganic & Medicinal Chemistry. 2012, 20(7), pp. 2416-2418. ISSN 0968-0896. eISSN 1464-3391. Available under: doi: 10.1016/j.bmc.2012.01.044BibTex
@article{Santner2012-04-01synth-18857,
year={2012},
doi={10.1016/j.bmc.2012.01.044},
title={The synthesis of 2′-methylseleno adenosine and guanosine 5′-triphosphates},
number={7},
volume={20},
issn={0968-0896},
journal={Bioorganic & Medicinal Chemistry},
pages={2416--2418},
author={Santner, Tobias and Siegmund, Vanessa and Marx, Andreas and Micura, Ronald}
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<dcterms:abstract xml:lang="eng">Modified nucleoside triphosphates (NTPs) represent powerful building blocks to generate nucleic acids with novel properties by enzymatic synthesis. We have recently demonstrated the access to 20-SeCH3-uridine and 20-SeCH3-cytidine derivatized RNAs for applications in RNA crystallography, using the corresponding<br />nucleoside triphosphates and distinct mutants of T7 RNA polymerase. In the present note, we introduce the chemical synthesis of the novel 20-methylseleno-20-deoxyadenosine and -guanosine 50-triphosphates (20-SeCH3-ATP and 20-SeCH3-GTP) that represent further candidates for the enzymatic RNA synthesis with engineered RNA polymerases.</dcterms:abstract>
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