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Exo-endocytosis in isolated peptidergic nerve terminals occurs in the sub-second range

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1992

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Knoll, Gerd
Nordmann, Jean J.

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Bioscience Reports. Portland Press. 1992, 12(6), pp. 495-501. ISSN 0144-8463. eISSN 1573-4935. Available under: doi: 10.1007/BF01122037

Zusammenfassung

Exo- and endocytotic processes induced by depolarization of isolated neurosecretory nerve terminals show a close temporal correlation, which suggests a short time of integration of the neurosecretory granule membrane with the plasma membrane. In order to determine minimal time requirements for exocytosis-coupled endocytosis to occur, we have analyzed by electron microscopy uptake of horseradish peroxidase (HRP) as a fluid phase marker at the onset of depolarization. We have applied rapid mixing and sampling (quenched flow) to assess events in sub-second time periods after stimulation. A significant number of labelled endocytotic vacuoles was observed during the first second of depolarization. This number then further increased by a factor of about 2 (within 5 s) and 4 (within 50 s). Thus, as for exocytosis, the rate of endocytosis decreased considerably during prolonged stimulation. These data indicate i) that a substantial proportion of secretory granules undergoes exocytosis very shortly after stimulation, and ii) that, following exocytosis, the minimal time required for consecutive membrane retrieval is in the sub-second range.

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570 Biowissenschaften, Biologie

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Electron microscopy, secretion, neuropeptides, exocytosis, endocytosis

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ISO 690KNOLL, Gerd, Helmut PLATTNER, Jean J. NORDMANN, 1992. Exo-endocytosis in isolated peptidergic nerve terminals occurs in the sub-second range. In: Bioscience Reports. Portland Press. 1992, 12(6), pp. 495-501. ISSN 0144-8463. eISSN 1573-4935. Available under: doi: 10.1007/BF01122037
BibTex
@article{Knoll1992-12Exoen-55089,
  year={1992},
  doi={10.1007/BF01122037},
  title={Exo-endocytosis in isolated peptidergic nerve terminals occurs in the sub-second range},
  number={6},
  volume={12},
  issn={0144-8463},
  journal={Bioscience Reports},
  pages={495--501},
  author={Knoll, Gerd and Plattner, Helmut and Nordmann, Jean J.}
}
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