Publikation:

Chemical proteomics reveals interactors of the alarmone diadenosine triphosphate in the cancer cell line H1299

Lade...
Vorschaubild

Dateien

Albrecht_2-1frtdfe6tc2mj5.pdf
Albrecht_2-1frtdfe6tc2mj5.pdfGröße: 1.11 MBDownloads: 17

Datum

2023

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

DOI (zitierfähiger Link)
ArXiv-ID

Internationale Patentnummer

Link zur Lizenz

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Hybrid
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Journal of Peptide Science. Wiley. 2023, 29(3), e3458. ISSN 1075-2617. eISSN 1099-1387. Available under: doi: 10.1002/psc.3458

Zusammenfassung

Intracellular dinucleoside polyphosphates (Npn Ns) have been known for decades but the functional role remains enigmatic. Diadenosine triphosphate (Ap3 A) is one of the most prominent examples, and its intercellular concentration was shown to increase upon cellular stress. By employment of previously reported Ap3 A-based photoaffinity-labeling probes (PALPs) in chemical proteomics, we investigated the Ap3 A interactome in the human lung carcinoma cell line H1299. The cell line is deficient of the fragile histidine triade (Fhit) protein, a hydrolase of Ap3 A and tumor suppressor. Overall, the number of identified potential interaction partners was significantly lower than in the previously investigated HEK293T cell line. Gene ontology term analysis revealed that the identified proteins participate in similar pathways as for HEK293T, but the percentage of proteins involved in RNA-related processes is higher for H1299. The obtained results highlight similarities and differences of the Ap3 A interaction network in different cell lines and give further indications regarding the importance of the presence of Fhit.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
540 Chemie

Schlagwörter

alarmone, Ap3A, nucleotide, photoaffinity labeling, proteomics

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690ALBRECHT, Christoph J., Florian M. STUMPF, Lena KRÜGER, Marie L. NIEDERMEIER, Florian STENGEL, Andreas MARX, 2023. Chemical proteomics reveals interactors of the alarmone diadenosine triphosphate in the cancer cell line H1299. In: Journal of Peptide Science. Wiley. 2023, 29(3), e3458. ISSN 1075-2617. eISSN 1099-1387. Available under: doi: 10.1002/psc.3458
BibTex
@article{Albrecht2023Chemi-59109,
  year={2023},
  doi={10.1002/psc.3458},
  title={Chemical proteomics reveals interactors of the alarmone diadenosine triphosphate in the cancer cell line H1299},
  number={3},
  volume={29},
  issn={1075-2617},
  journal={Journal of Peptide Science},
  author={Albrecht, Christoph J. and Stumpf, Florian M. and Krüger, Lena and Niedermeier, Marie L. and Stengel, Florian and Marx, Andreas},
  note={Article Number: e3458}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/59109">
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dc:contributor>Stumpf, Florian M.</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:title>Chemical proteomics reveals interactors of the alarmone diadenosine triphosphate in the cancer cell line H1299</dcterms:title>
    <dcterms:issued>2023</dcterms:issued>
    <dc:creator>Krüger, Lena</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-11-10T14:08:19Z</dc:date>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/59109/1/Albrecht_2-1frtdfe6tc2mj5.pdf"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Niedermeier, Marie L.</dc:contributor>
    <dc:contributor>Stengel, Florian</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-11-10T14:08:19Z</dcterms:available>
    <dc:contributor>Albrecht, Christoph J.</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/59109"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Niedermeier, Marie L.</dc:creator>
    <dc:creator>Stumpf, Florian M.</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Marx, Andreas</dc:contributor>
    <dc:creator>Albrecht, Christoph J.</dc:creator>
    <dcterms:abstract xml:lang="eng">Intracellular dinucleoside polyphosphates (Np&lt;sub&gt;n&lt;/sub&gt; Ns) have been known for decades but the functional role remains enigmatic. Diadenosine triphosphate (Ap&lt;sub&gt;3&lt;/sub&gt; A) is one of the most prominent examples, and its intercellular concentration was shown to increase upon cellular stress. By employment of previously reported Ap3 A-based photoaffinity-labeling probes (PALPs) in chemical proteomics, we investigated the Ap3 A interactome in the human lung carcinoma cell line H1299. The cell line is deficient of the fragile histidine triade (Fhit) protein, a hydrolase of Ap&lt;sub&gt;3&lt;/sub&gt; A and tumor suppressor. Overall, the number of identified potential interaction partners was significantly lower than in the previously investigated HEK293T cell line. Gene ontology term analysis revealed that the identified proteins participate in similar pathways as for HEK293T, but the percentage of proteins involved in RNA-related processes is higher for H1299. The obtained results highlight similarities and differences of the Ap3 A interaction network in different cell lines and give further indications regarding the importance of the presence of Fhit.</dcterms:abstract>
    <dc:creator>Marx, Andreas</dc:creator>
    <dc:language>eng</dc:language>
    <dc:contributor>Krüger, Lena</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Stengel, Florian</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/59109/1/Albrecht_2-1frtdfe6tc2mj5.pdf"/>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
Diese Publikation teilen