Energetics and biochemistry of fermentative benzoate degradation by Syntrophus gentianae

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1999
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Schöcke, Ludger
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Archives of Microbiology. 1999, 171(5), pp. 331-337. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s002030050718
Zusammenfassung

The pathway of fermentative benzoate degradation by the syntrophically fermenting bacterium Syntrophus gentianae was studied by measurement of enzyme activities in cell-free extracts. Benzoate was activated by a benzoate-CoA ligase reaction, forming AMP and pyrophosphate, which was subsequently cleaved by a membrane-bound proton-translocating pyrophosphatase. Glutaconyl-CoA (formed from hypothetical pimelyl-CoA and glutaryl-CoA intermediates) was decarboxylated to crotonyl-CoA by a sodium-ion-dependent membrane-bound glutaconyl-CoA decarboxylase, a biotin enzyme that could be inhibited by avidin. The overall energy budget of this fermentation could be balanced only if the dearomatizing reduction of benzoyl-CoA is assumed to produce cyclohexene carboxyl-CoA rather than cyclohexadiene carboxyl-CoA, although experimental evidence of this reaction is still insufficient. With this assumption, benzoate degradation by S. gentianae can be balanced to yield onethird to two-thirds of an ATP unit per benzoate degraded, in accordance with earlier measurements of whole-cell energetics.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Syntrophus gentianae, Syntrophic benzoate degradation, Glutaconyl-CoA decarboxylase, Proton-translocating pyrophosphatase, Benzoyl-CoA
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ISO 690SCHÖCKE, Ludger, Bernhard SCHINK, 1999. Energetics and biochemistry of fermentative benzoate degradation by Syntrophus gentianae. In: Archives of Microbiology. 1999, 171(5), pp. 331-337. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s002030050718
BibTex
@article{Schocke1999Energ-6564,
  year={1999},
  doi={10.1007/s002030050718},
  title={Energetics and biochemistry of fermentative benzoate degradation by Syntrophus gentianae},
  number={5},
  volume={171},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={331--337},
  author={Schöcke, Ludger and Schink, Bernhard}
}
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