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Molecular cloning of cDNA for rat liver general acyl CoA dehydrogenase and homology between the rat liver and pig kidney enzymes

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Molecular_cloning_of_cDNA_for_rat_liver_general_acyl_CoA_dehydrogenase_and_homology_between_the_rat_liver_and_pig_kidney_enzymes.pdf
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Datum

1987

Autor:innen

Inagaki, Taisuke
Ohishi, Nobuko
Rasched, Ihab
Frank, Rainer W.
Tsukagoshi, Norihiro
Udaka, Shigezo
Yagi, Kunio

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http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-55487
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Attribution-NonCommercial-NoDerivs 2.0 Generic

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Biologie: Publikationen
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Published

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Biochemistry International. 1987, 15(5), pp. 1051-1056

Zusammenfassung

cDNA clone for general acyl CoA dehydrogenase (GAD) was isolated from a rat liver cDNA expression library in λgtll using anti-pig kidney GAD antibody. Size of the isolated cDNA was estimated to be 1.5-1.6 kb. By immunological analysis of fusion protein and epitope selection, the cDNA clone was identified as that containing the GAD gene. Partial amino acid sequence deduced from nucleotide sequence of the cDNA coincided with that of the pig kidney enzyme. The antibody cross-reacted with rat liver enzyme and molecular weights of these enzyme proteins were shown to be almost the same. All these results indicate that rat liver GAD shares a common structure with pig kidney enzyme.

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570 Biowissenschaften, Biologie

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ISO 690INAGAKI, Taisuke, Nobuko OHISHI, Ihab RASCHED, Rainer W. FRANK, Sandro GHISLA, Norihiro TSUKAGOSHI, Shigezo UDAKA, Kunio YAGI, 1987. Molecular cloning of cDNA for rat liver general acyl CoA dehydrogenase and homology between the rat liver and pig kidney enzymes. In: Biochemistry International. 1987, 15(5), pp. 1051-1056
BibTex
@article{Inagaki1987Molec-7257,
  year={1987},
  title={Molecular cloning of cDNA for rat liver general acyl CoA dehydrogenase and homology between the rat liver and pig kidney enzymes},
  number={5},
  volume={15},
  journal={Biochemistry International},
  pages={1051--1056},
  author={Inagaki, Taisuke and Ohishi, Nobuko and Rasched, Ihab and Frank, Rainer W. and Ghisla, Sandro and Tsukagoshi, Norihiro and Udaka, Shigezo and Yagi, Kunio}
}
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