Publikation: Ca2+ release from subplasmalemmal stores as a primary event during exocytosis in Paramecium cells
Lade...
Dateien
Datum
1994
Autor:innen
Erxleben, Christian
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Journal of Cell Biology. 1994, 127(4), pp. 935-945. ISSN 0021-9525. eISSN 1540-8140. Available under: doi: 10.1083/jcb.127.4.935
Zusammenfassung
A correlated electrophysiological and light microscopic evaluation of trichocyst exocytosis was carded out with Paramecium cells which possess extensive cortical Ca stores with footlike links to the plasmalemma. We used not only intra- but also extracellular recordings to account for polar arrangement of ion channels (while trichocysts can be released from all over the cell surface). With three widely different secretagogues, aminoethyldextran (AED), veratridine and caffeine, similar anterior Nain and posterior Kout currents (both known to be Ca 2+-dependent) were observed. Direct de- or hyperpolarization induced by current injection failed to trigger exocytosis. For both, exocytotic membrane fusion and secretagogue-induced membrane currents, sensitivity to or availability of Ca2+ appears to be different. Current responses to AED were blocked by W7 or trifluoperazine, while exocytosis remained unaffected. Reducing [Ca2+]o to
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690
ERXLEBEN, Christian, Helmut PLATTNER, 1994. Ca2+ release from subplasmalemmal stores as a primary event during exocytosis in Paramecium cells. In: Journal of Cell Biology. 1994, 127(4), pp. 935-945. ISSN 0021-9525. eISSN 1540-8140. Available under: doi: 10.1083/jcb.127.4.935BibTex
@article{Erxleben1994relea-8463,
year={1994},
doi={10.1083/jcb.127.4.935},
title={Ca2+ release from subplasmalemmal stores as a primary event during exocytosis in Paramecium cells},
number={4},
volume={127},
issn={0021-9525},
journal={Journal of Cell Biology},
pages={935--945},
author={Erxleben, Christian and Plattner, Helmut}
}RDF
<rdf:RDF
xmlns:dcterms="http://purl.org/dc/terms/"
xmlns:dc="http://purl.org/dc/elements/1.1/"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:bibo="http://purl.org/ontology/bibo/"
xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
xmlns:foaf="http://xmlns.com/foaf/0.1/"
xmlns:void="http://rdfs.org/ns/void#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#" >
<rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8463">
<dc:creator>Erxleben, Christian</dc:creator>
<dc:contributor>Plattner, Helmut</dc:contributor>
<void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
<dcterms:title>Ca2+ release from subplasmalemmal stores as a primary event during exocytosis in Paramecium cells</dcterms:title>
<foaf:homepage rdf:resource="http://localhost:8080/"/>
<dcterms:issued>1994</dcterms:issued>
<dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8463/1/Ca2_release_from_subplasmalemmal_stores_as_a_primary_event_during_exocytosis_in_Paramecium_cells.pdf"/>
<dcterms:bibliographicCitation>First publ. in: Journal of Cell Biology 127 (1994), 4, pp. 935-945</dcterms:bibliographicCitation>
<dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
<dc:language>eng</dc:language>
<dc:contributor>Erxleben, Christian</dc:contributor>
<dc:format>application/pdf</dc:format>
<dcterms:abstract xml:lang="eng">A correlated electrophysiological and light microscopic evaluation of trichocyst exocytosis was carded out with Paramecium cells which possess extensive cortical Ca stores with footlike links to the plasmalemma. We used not only intra- but also extracellular recordings to account for polar arrangement of ion channels (while trichocysts can be released from all over the cell surface). With three widely different secretagogues, aminoethyldextran (AED), veratridine and caffeine, similar anterior Nain and posterior Kout currents (both known to be Ca 2+-dependent) were observed. Direct de- or hyperpolarization induced by current injection failed to trigger exocytosis. For both, exocytotic membrane fusion and secretagogue-induced membrane currents, sensitivity to or availability of Ca2+ appears to be different. Current responses to AED were blocked by W7 or trifluoperazine, while exocytosis remained unaffected. Reducing [Ca2+]o to</dcterms:abstract>
<dc:creator>Plattner, Helmut</dc:creator>
<dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
<dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8463/1/Ca2_release_from_subplasmalemmal_stores_as_a_primary_event_during_exocytosis_in_Paramecium_cells.pdf"/>
<bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8463"/>
<dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:51Z</dc:date>
<dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
<dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:51Z</dcterms:available>
<dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
</rdf:Description>
</rdf:RDF>
