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A Knockout of the Photoreceptor PtAureo1a Results in Altered Diel Expression of Diatom Clock Components

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2024

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Deutsche Forschungsgemeinschaft (DFG): KR 1661-18
Deutsche Forschungsgemeinschaft (DFG): KR 1661-20
Deutsche Forschungsgemeinschaft (DFG): LE 3358/3

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Plants. MDPI. 2024, 13(11), 1465. eISSN 2223-7747. Verfügbar unter: doi: 10.3390/plants13111465

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Plants and algae use light not only for driving photosynthesis but also to sense environmental cues and to adjust their circadian clocks via photoreceptors. Aureochromes are blue-light-dependent photoreceptors that also function as transcription factors, possessing both a LOV and a bZIP domain. Aureochromes so far have only been detected in Stramenopile algae, which include the diatoms. Four paralogues of aureochromes have been identified in the pennate model diatom Phaeodactylum tricornutum: PtAureo1a, 1b, 1c, and 2. While it was shown recently that diatoms have a diel rhythm, the molecular mechanisms and components regulating it are still largely unknown. Diel gene expression analyses of wild-type P. tricornutum, a PtAureo1a knockout strain, and the respective PtAureo1 complemented line revealed that all four aureochromes have a different diel regulation and that PtAureo1a has a strong co-regulatory influence on its own transcription, as well as on that of other genes encoding different blue-light photoreceptors (CPF1, 2 and 4), proteins involved in photoprotection (Lhcx1), and specific bHLH transcription factors (RITMO1). Some of these genes completely lost their circadian expression in the PtAureo1a KO mutant. Our results suggest a major involvement of aureochromes in the molecular clock of diatoms.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie

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diatoms, blue light, photoreceptor, circadian clock, mRNA expression analysis, diel regulation

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ISO 690MADHURI, Shvaita, Bernard LEPETIT, Alexander Helmut FÜRST, Peter G. KROTH, 2024. A Knockout of the Photoreceptor PtAureo1a Results in Altered Diel Expression of Diatom Clock Components. In: Plants. MDPI. 2024, 13(11), 1465. eISSN 2223-7747. Verfügbar unter: doi: 10.3390/plants13111465
BibTex
@article{Madhuri2024Knock-70271,
  year={2024},
  doi={10.3390/plants13111465},
  title={A Knockout of the Photoreceptor PtAureo1a Results in Altered Diel Expression of Diatom Clock Components},
  number={11},
  volume={13},
  journal={Plants},
  author={Madhuri, Shvaita and Lepetit, Bernard and Fürst, Alexander Helmut and Kroth, Peter G.},
  note={Article Number: 1465}
}
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