Publikation: Fluorescence Properties of Reduced Flavins and Flavoproteins
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1979
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European Journal of Biochemistry. 1979, 101(1), pp. 13-21. ISSN 0014-2956. eISSN 1432-1033. Available under: doi: 10.1111/j.1432-1033.1979.tb04210.x
Zusammenfassung
Fluorescence lifetimes and polarized emission properties of reduced flavin were measured using several model compounds and flavoproteins. Depending on the conditions of solvent and temperature or reduction method the lifetimes vary between 1 and 15 ns. The longer lifetime values are found in several forms of reduced lactate oxidase, in which a good correlation exists between fluorescence intensity and lifetime. In practically all flavoproteins the fluorescence is heterogeneous. Several mechanisms are proposed to explain the observed heterogeneity in lifetimes. The reduced models in glycerol at subzero temperature exhibit high degrees of polarization of the fluorescence, whereas distinct depolarization is encountered in several reduced flavoproteins suggesting a certain mobility of the flavin chromophor.
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570 Biowissenschaften, Biologie
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VISSER, Antonie J. W. G., Sandro GHISLA, Vincent MASSEY, Franz MÜLLER, Cees VEEGER, 1979. Fluorescence Properties of Reduced Flavins and Flavoproteins. In: European Journal of Biochemistry. 1979, 101(1), pp. 13-21. ISSN 0014-2956. eISSN 1432-1033. Available under: doi: 10.1111/j.1432-1033.1979.tb04210.xBibTex
@article{Visser1979Fluor-7177,
year={1979},
doi={10.1111/j.1432-1033.1979.tb04210.x},
title={Fluorescence Properties of Reduced Flavins and Flavoproteins},
number={1},
volume={101},
issn={0014-2956},
journal={European Journal of Biochemistry},
pages={13--21},
author={Visser, Antonie J. W. G. and Ghisla, Sandro and Massey, Vincent and Müller, Franz and Veeger, Cees}
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