Publikation: Fermentative degradation of triethanolamine by a homoacetogenic bacterium
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With triethanolamine as sole source of energy and organic carbon, a strictly anaerobic, gram-positive, rod-shaped bacterium, strain LuTria 3, was isolated from sewage sludge and was assigned to the genus Acetobacterium on the basis of morphological and physiological properties. The G + C content of the DNA was 34.9 + 1.0 mol %. The new isolate fermented triethanolamine to acetate and ammonia. In cell-free extracts, a triethanolaminedegrading enzyme activity was detected that formed acetaldehyde as reaction product. Triethanolamine cleavage was stimulated 30-fold by added adenosylcobalamin (coenzyme B12) and inhibited by cyanocobalamin or hydroxocobalamin. Ethanolamine ammonia lyase, cetaldehyde:acceptor oxidoreductase, phosphate acetyltransferase, acetate kinase, and carbon monoxide dehydrogenase were measured in cell-free extracts of this strain. Our results establish that triethanolamine is degraded by a orrinoid-dependent shifting of the terminal hydroxyl group to the subterminal carbon atom, analogous to a diol dehydratase reaction, to form an unstable intermediate that releases acetaldehyde. No anaerobic degradation of triethylamine was observed in similar enrichment assays.
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FRINGS, Joachim, Christine WONDRAK, Bernhard SCHINK, 1994. Fermentative degradation of triethanolamine by a homoacetogenic bacterium. In: Archives of Microbiology. 1994, 162(1-2), pp. 103-107. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00264381BibTex
@article{Frings1994Ferme-8492, year={1994}, doi={10.1007/BF00264381}, title={Fermentative degradation of triethanolamine by a homoacetogenic bacterium}, number={1-2}, volume={162}, issn={0302-8933}, journal={Archives of Microbiology}, pages={103--107}, author={Frings, Joachim and Wondrak, Christine and Schink, Bernhard} }
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