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Fermentative degradation of triethanolamine by a homoacetogenic bacterium

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1994

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Frings, Joachim
Wondrak, Christine

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Archives of Microbiology. 1994, 162(1-2), pp. 103-107. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00264381

Zusammenfassung

With triethanolamine as sole source of energy and organic carbon, a strictly anaerobic, gram-positive, rod-shaped bacterium, strain LuTria 3, was isolated from sewage sludge and was assigned to the genus Acetobacterium on the basis of morphological and physiological properties. The G + C content of the DNA was 34.9 + 1.0 mol %. The new isolate fermented triethanolamine to acetate and ammonia. In cell-free extracts, a triethanolaminedegrading enzyme activity was detected that formed acetaldehyde as reaction product. Triethanolamine cleavage was stimulated 30-fold by added adenosylcobalamin (coenzyme B12) and inhibited by cyanocobalamin or hydroxocobalamin. Ethanolamine ammonia lyase, cetaldehyde:acceptor oxidoreductase, phosphate acetyltransferase, acetate kinase, and carbon monoxide dehydrogenase were measured in cell-free extracts of this strain. Our results establish that triethanolamine is degraded by a orrinoid-dependent shifting of the terminal hydroxyl group to the subterminal carbon atom, analogous to a diol dehydratase reaction, to form an unstable intermediate that releases acetaldehyde. No anaerobic degradation of triethylamine was observed in similar enrichment assays.

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570 Biowissenschaften, Biologie

Schlagwörter

Anaerobic degradation, Triethanolamine Homoacetogenic fermentation, Corrinoids, Triethanolamine degrading enzyme

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ISO 690FRINGS, Joachim, Christine WONDRAK, Bernhard SCHINK, 1994. Fermentative degradation of triethanolamine by a homoacetogenic bacterium. In: Archives of Microbiology. 1994, 162(1-2), pp. 103-107. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00264381
BibTex
@article{Frings1994Ferme-8492,
  year={1994},
  doi={10.1007/BF00264381},
  title={Fermentative degradation of triethanolamine by a homoacetogenic bacterium},
  number={1-2},
  volume={162},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={103--107},
  author={Frings, Joachim and Wondrak, Christine and Schink, Bernhard}
}
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