Publikation:

Ion Selectivity of the Cytoplasmic Binding Sites of the Na,K-ATPase: II. Competition of Various Cations

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2001

Autor:innen

Schneeberger, Anne

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Published

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The Journal of Membrane Biology. 2001, 179(3), pp. 263-273. ISSN 0022-2631. eISSN 1432-1424. Available under: doi: 10.1007/s002320010051

Zusammenfassung

In the E1 state of the Na,K-ATPase all cations present in the cytoplasm compete for the ion binding sites. The mutual effects of mono-, di- and trivalent cations were investigated by experiments with the electrochromic fluorescent dye RH421. Three sites with significantly different properties could be identified. The most unspecific binding site is able to bind all cations, independent of their valence and size. The large organic cation Br2-Titu3+ is bound with the highest affinity ( Na+ > Cs+ > Li+. The second type of binding site is specific for monovalent cations, its binding affinity is higher than that of the first type, for Na+ ions the equilibrium dissociation constant is < 0.01 mM. Since binding to that site is not electrogenic it has to be close to the cytoplasmic surface. The third site is specific for Na+, no other ions were found to bind, the binding is electrogenic and the equilibrium dissociation constant is 0.2 mM.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Sodium pump, Cytoplasmic ion binding, Electrochromic fluorescent dye, Ion transport, Monovalent ions, Magnesium effects

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ISO 690SCHNEEBERGER, Anne, Hans-Jürgen APELL, 2001. Ion Selectivity of the Cytoplasmic Binding Sites of the Na,K-ATPase: II. Competition of Various Cations. In: The Journal of Membrane Biology. 2001, 179(3), pp. 263-273. ISSN 0022-2631. eISSN 1432-1424. Available under: doi: 10.1007/s002320010051
BibTex
@article{Schneeberger2001Selec-8400,
  year={2001},
  doi={10.1007/s002320010051},
  title={Ion Selectivity of the Cytoplasmic Binding Sites of the Na,K-ATPase: II. Competition of Various Cations},
  number={3},
  volume={179},
  issn={0022-2631},
  journal={The Journal of Membrane Biology},
  pages={263--273},
  author={Schneeberger, Anne and Apell, Hans-Jürgen}
}
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