Publikation: Genome editing in East African cichlids and tilapias : state-of-the-art and future directions
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African cichlid fishes of the Cichlidae family are a group of teleosts important for aquaculture and research. A thriving research community is particularly interested in the cichlid radiations of the East African Great Lakes. One key goal is to pinpoint genetic variation underlying phenotypic diversification, but the lack of genetic tools has precluded thorough dissection of the genetic basis of relevant traits in cichlids. Genome editing technologies are well established in teleost models like zebrafish and medaka. However, this is not the case for emerging model organisms, such as East African cichlids, where these technologies remain inaccessible to most laboratories, due in part to limited exchange of knowledge and expertise. The Cichlid Science 2022 meeting (Cambridge, UK) hosted for the first time a Genome Editing Workshop, where the community discussed recent advances in genome editing, with an emphasis on CRISPR/Cas9 technologies. Based on the workshop findings and discussions, in this review we define the state-of-the-art of cichlid genome editing, share resources and protocols, and propose new possible avenues to further expand the cichlid genome editing toolkit.
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CLARK, Bethan, Muktai KUWALEKAR, Bettina FISCHER, Joost M. WOLTERING, Jakob BIRAN, Scott JUNTTI, Claudius F. KRATOCHWIL, M. Emília SANTOS, Miguel VASCONCELOS ALMEIDA, 2023. Genome editing in East African cichlids and tilapias : state-of-the-art and future directions. In: Open Biology. Royal Society of London. 2023, 13(11), 230257. eISSN 2046-2441. Available under: doi: 10.1098/rsob.230257BibTex
@article{Clark2023Genom-68795, year={2023}, doi={10.1098/rsob.230257}, title={Genome editing in East African cichlids and tilapias : state-of-the-art and future directions}, number={11}, volume={13}, journal={Open Biology}, author={Clark, Bethan and Kuwalekar, Muktai and Fischer, Bettina and Woltering, Joost M. and Biran, Jakob and Juntti, Scott and Kratochwil, Claudius F. and Santos, M. Emília and Vasconcelos Almeida, Miguel}, note={Article Number: 230257} }
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