Publikation: A Systematic Analysis of Mosquito-Microbiome Biosynthetic Gene Clusters Reveals Antimalarial Siderophores that Reduce Mosquito Reproduction Capacity
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Advances in infectious disease control strategies through genetic manipulation of insect microbiomes have heightened interest in microbially produced small molecules within mosquitoes. Herein, 33 mosquito-associated bacterial genomes were mined and over 700 putative biosynthetic gene clusters (BGCs) were identified, 135 of which belong to known classes of BGCs. After an in-depth analysis of the 135 BGCs, iron-binding siderophores were chosen for further investigation due to their high abundance and well-characterized bioactivities. Through various metabolomic strategies, eight siderophore scaffolds were identified in six strains of mosquito-associated bacteria. Among these, serratiochelin A and pyochelin were found to reduce female Anopheles gambiae overall fecundity likely by lowering their blood-feeding rate. Serratiochelin A and pyochelin were further found to inhibit the Plasmodium parasite asexual blood and liver stages in vitro. Our work supplies a bioinformatic resource for future mosquito-microbiome studies and highlights an understudied source of bioactive small molecules.
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GANLEY, Jack G., Ashmita PANDEY, Kayla SYLVESTER, Kuan-Yi LU, Maria TORO-MORENO, Sina RÜTSCHLIN, James M. BRADFORD, Cody J. CHAMPION, Thomas BÖTTCHER, Jiannong XU, Emily R. DERBYSHIRE, 2020. A Systematic Analysis of Mosquito-Microbiome Biosynthetic Gene Clusters Reveals Antimalarial Siderophores that Reduce Mosquito Reproduction Capacity. In: Cell Chemical Biology. Cell Press. 2020, 27(7), pp. 817-826.e5. ISSN 2451-9456. eISSN 2451-9448. Available under: doi: 10.1016/j.chembiol.2020.06.004BibTex
@article{Ganley2020-07-16Syste-50832, year={2020}, doi={10.1016/j.chembiol.2020.06.004}, title={A Systematic Analysis of Mosquito-Microbiome Biosynthetic Gene Clusters Reveals Antimalarial Siderophores that Reduce Mosquito Reproduction Capacity}, number={7}, volume={27}, issn={2451-9456}, journal={Cell Chemical Biology}, pages={817--826.e5}, author={Ganley, Jack G. and Pandey, Ashmita and Sylvester, Kayla and Lu, Kuan-Yi and Toro-Moreno, Maria and Rütschlin, Sina and Bradford, James M. and Champion, Cody J. and Böttcher, Thomas and Xu, Jiannong and Derbyshire, Emily R.} }
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