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Assessing Genotoxicity of Ten Different Engineered Nanomaterials by the Novel Semi-Automated FADU Assay and the Alkaline Comet Assay

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2022

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Hirsch, Cordula
Rippl, Alexandra
Wick, Peter

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Open Access Gold
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Published

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Nanomaterials. MDPI. 2022, 12(2), 220. eISSN 2079-4991. Available under: doi: 10.3390/nano12020220

Zusammenfassung

Increased engineered nanomaterial (ENM) production and incorporation in consumer and biomedical products has raised concerns about the potential adverse effects. The DNA damaging capacity is of particular importance since damaged genetic material can lead to carcinogenesis. Consequently, reliable and robust in vitro studies assessing ENM genotoxicity are of great value. We utilized two complementary assays based on different measurement principles: (1) comet assay and (2) FADU (fluorimetric detection of alkaline DNA unwinding) assay. Assessing cell viability ruled out false-positive results due to DNA fragmentation during cell death. Potential structure–activity relationships of 10 ENMs were investigated: three silica nanoparticles (SiO2-NP) with varying degrees of porosity, titanium dioxide (TiO2-NP), polystyrene (PS-NP), zinc oxide (ZnO-NP), gold (Au-NP), graphene oxide (GO) and two multi-walled carbon nanotubes (MWNT). SiO2-NPs, TiO2-NP and GO were neither cytotoxic nor genotoxic to Jurkat E6-I cells. Quantitative interference corrections derived from GO results can make the FADU assay a promising screening tool for a variety of ENMs. MWNT merely induced cytotoxicity, while dose- and time-dependent cytotoxicity of PS-NP was accompanied by DNA fragmentation. Hence, PS-NP served to benchmark threshold levels of cytotoxicity at which DNA fragmentation was expected. Considering all controls revealed the true genotoxicity for Au-NP and ZnO-NP at early time Points.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

comet assay; FADU assay; engineered nanomaterials; DNA strand breaks; genotoxicity; ENM interference

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ISO 690MAY, Sarah, Cordula HIRSCH, Alexandra RIPPL, Alexander BÜRKLE, Peter WICK, 2022. Assessing Genotoxicity of Ten Different Engineered Nanomaterials by the Novel Semi-Automated FADU Assay and the Alkaline Comet Assay. In: Nanomaterials. MDPI. 2022, 12(2), 220. eISSN 2079-4991. Available under: doi: 10.3390/nano12020220
BibTex
@article{May2022-01-10Asses-56415,
  year={2022},
  doi={10.3390/nano12020220},
  title={Assessing Genotoxicity of Ten Different Engineered Nanomaterials by the Novel Semi-Automated FADU Assay and the Alkaline Comet Assay},
  number={2},
  volume={12},
  journal={Nanomaterials},
  author={May, Sarah and Hirsch, Cordula and Rippl, Alexandra and Bürkle, Alexander and Wick, Peter},
  note={Article Number: 220}
}
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