Publikation:

Self-Collected Gargle Lavage Allows Reliable Detection of SARS-CoV-2 in an Outpatient Setting

Lade...
Vorschaubild

Dateien

Zander_2-17rmqtqagqkhk8.pdf
Zander_2-17rmqtqagqkhk8.pdfGröße: 267.22 KBDownloads: 93

Datum

2021

Autor:innen

Zander, Johannes
Scholtes, Stephan
Ottinger, Maximilian
Kharazi, Azadeh
Bickmann, Julia
Zeleny, Christian

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Link zur Lizenz

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Hybrid
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Microbiology Spectrum. American Society for Microbiology (ASM). 2021, 9(1), e00361-21. eISSN 2165-0497. Available under: doi: 10.1128/Spectrum.00361-21

Zusammenfassung

Current procurement of specimens for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection requires trained personnel and dedicated equipment. We compared standard nasopharyngeal swabs with self-collected gargle lavage fluid obtained from 80 mostly symptomatic outpatients. After RNA extraction, RT-PCR to detect SARS-CoV-2 was performed. Qualitative results obtained with the paired samples from individual outpatients were 100% congruent. Therefore, self-collected gargle lavage fluid can serve as a suitable specimen for coronavirus disease 2019 (COVID-19) testing in outpatients.

IMPORTANCE The SARS-CoV-2 pandemic still strains health care systems worldwide. While COVID-19 testing is considered an essential pillar in combating this infectious disease, shortages in supplies and trained health care personnel often limit the procurement of patient samples, in particular in outpatient settings. Here, we compared the simple self-collection of gargle lavage fluid with the gold standard nasopharyngeal swab as a specimen for COVID-19 testing. By finding complete congruence of results obtained with paired samples of a sizeable patient cohort, our results strongly support the idea that the painless self-collection of gargle lavage fluid provides a suitable and uncomplicated sample for reliable SARS-CoV-2 detection.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

COVID-19, RT-PCR, SARS-CoV-2, gargle lavage, outpatients

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690ZANDER, Johannes, Stephan SCHOLTES, Maximilian OTTINGER, Marcel KREMER, Azadeh KHARAZI, Vanessa STADLER, Julia BICKMANN, Christian ZELENY, Johannes W. P. KUIPER, Christof R. HAUCK, 2021. Self-Collected Gargle Lavage Allows Reliable Detection of SARS-CoV-2 in an Outpatient Setting. In: Microbiology Spectrum. American Society for Microbiology (ASM). 2021, 9(1), e00361-21. eISSN 2165-0497. Available under: doi: 10.1128/Spectrum.00361-21
BibTex
@article{Zander2021SelfC-55308,
  year={2021},
  doi={10.1128/Spectrum.00361-21},
  title={Self-Collected Gargle Lavage Allows Reliable Detection of SARS-CoV-2 in an Outpatient Setting},
  number={1},
  volume={9},
  journal={Microbiology Spectrum},
  author={Zander, Johannes and Scholtes, Stephan and Ottinger, Maximilian and Kremer, Marcel and Kharazi, Azadeh and Stadler, Vanessa and Bickmann, Julia and Zeleny, Christian and Kuiper, Johannes W. P. and Hauck, Christof R.},
  note={Article Number: e00361-21}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/55308">
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-10-20T11:31:45Z</dcterms:available>
    <dc:contributor>Zander, Johannes</dc:contributor>
    <dcterms:title>Self-Collected Gargle Lavage Allows Reliable Detection of SARS-CoV-2 in an Outpatient Setting</dcterms:title>
    <dc:creator>Zeleny, Christian</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:abstract xml:lang="eng">Current procurement of specimens for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection requires trained personnel and dedicated equipment. We compared standard nasopharyngeal swabs with self-collected gargle lavage fluid obtained from 80 mostly symptomatic outpatients. After RNA extraction, RT-PCR to detect SARS-CoV-2 was performed. Qualitative results obtained with the paired samples from individual outpatients were 100% congruent. Therefore, self-collected gargle lavage fluid can serve as a suitable specimen for coronavirus disease 2019 (COVID-19) testing in outpatients.&lt;br /&gt;&lt;br /&gt;IMPORTANCE The SARS-CoV-2 pandemic still strains health care systems worldwide. While COVID-19 testing is considered an essential pillar in combating this infectious disease, shortages in supplies and trained health care personnel often limit the procurement of patient samples, in particular in outpatient settings. Here, we compared the simple self-collection of gargle lavage fluid with the gold standard nasopharyngeal swab as a specimen for COVID-19 testing. By finding complete congruence of results obtained with paired samples of a sizeable patient cohort, our results strongly support the idea that the painless self-collection of gargle lavage fluid provides a suitable and uncomplicated sample for reliable SARS-CoV-2 detection.</dcterms:abstract>
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dc:contributor>Zeleny, Christian</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/55308/1/Zander_2-17rmqtqagqkhk8.pdf"/>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dc:language>eng</dc:language>
    <dc:contributor>Kremer, Marcel</dc:contributor>
    <dc:contributor>Scholtes, Stephan</dc:contributor>
    <dc:contributor>Kharazi, Azadeh</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Kuiper, Johannes W. P.</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Bickmann, Julia</dc:contributor>
    <dc:creator>Kremer, Marcel</dc:creator>
    <dc:creator>Zander, Johannes</dc:creator>
    <dc:creator>Hauck, Christof R.</dc:creator>
    <dc:creator>Ottinger, Maximilian</dc:creator>
    <dc:creator>Bickmann, Julia</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-10-20T11:31:45Z</dc:date>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/55308"/>
    <dc:contributor>Kuiper, Johannes W. P.</dc:contributor>
    <dc:creator>Scholtes, Stephan</dc:creator>
    <dc:creator>Stadler, Vanessa</dc:creator>
    <dcterms:issued>2021</dcterms:issued>
    <dc:contributor>Stadler, Vanessa</dc:contributor>
    <dc:contributor>Hauck, Christof R.</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/55308/1/Zander_2-17rmqtqagqkhk8.pdf"/>
    <dc:creator>Kharazi, Azadeh</dc:creator>
    <dc:contributor>Ottinger, Maximilian</dc:contributor>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Unbekannt
Diese Publikation teilen