Publikation: A BAC library of the east african haplochromine cichlid fish Astatotilapia burtoni
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A BAC library was constructed from Astatotilapia burtoni, a haplochromine cichlid that is found in Lake Tanganyika, East Africa, and its surrounding rivers. The library was generated from genomic DNA of blood cells and comprises 96,768 individual clones. Its median insert size is 150 kb and the coverage is expected to represent about 14 genome equivalents. The coverage evaluation was based on genome size estimates that were obtained by flow cytometry. In addition, hybridization screens with five probes largely corroborate the above coverage estimate, although the number of clones ranged from 5 to 22 authenticated clones per single copy probe. The BAC library described here is expected to be useful to the scientific community interested in cichlid genomics as an important resource to gain new insights into the rapid evolution of the great species diversity of haplochromine cichlid fishes.
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LANG, Michael, Tsutomu MIYAKE, Ingo BRAASCH, Deborah TINNEMORE, Nicol SIEGEL, Walter SALZBURGER, Chris T. AMEMIYA, Axel MEYER, 2006. A BAC library of the east african haplochromine cichlid fish Astatotilapia burtoni. In: Journal of experimental zoology / Part B, Molecular and developmental evolution. 2006, 306 B(Part B), pp. 35-44. ISSN 1552-5007. eISSN 1552-5015. Available under: doi: 10.1002/jez.b.21068BibTex
@article{Lang2006libra-6622,
year={2006},
doi={10.1002/jez.b.21068},
title={A BAC library of the east african haplochromine cichlid fish Astatotilapia burtoni},
number={Part B},
volume={306 B},
issn={1552-5007},
journal={Journal of experimental zoology / Part B, Molecular and developmental evolution},
pages={35--44},
author={Lang, Michael and Miyake, Tsutomu and Braasch, Ingo and Tinnemore, Deborah and Siegel, Nicol and Salzburger, Walter and Amemiya, Chris T. and Meyer, Axel}
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<dcterms:abstract xml:lang="eng">A BAC library was constructed from Astatotilapia burtoni, a haplochromine cichlid that is found in Lake Tanganyika, East Africa, and its surrounding rivers. The library was generated from genomic DNA of blood cells and comprises 96,768 individual clones. Its median insert size is 150 kb and the coverage is expected to represent about 14 genome equivalents. The coverage evaluation was based on genome size estimates that were obtained by flow cytometry. In addition, hybridization screens with five probes largely corroborate the above coverage estimate, although the number of clones ranged from 5 to 22 authenticated clones per single copy probe. The BAC library described here is expected to be useful to the scientific community interested in cichlid genomics as an important resource to gain new insights into the rapid evolution of the great species diversity of haplochromine cichlid fishes.</dcterms:abstract>
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