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Physical interaction between the strawberry allergen Fra a 1 and an associated partner FaAP : Interaction of Fra a 1 proteins and FaAP

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2017

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Franz-Oberdorf, Katrin
Langer, Andreas
Strasser, Ralf
Ranftl, Quirin L.
Wunschel, Christian
Schwab, Wilfried

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https://doi.org/10.1002/prot.25343
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Proteins : Structure, Function, and Bioinformatics. 2017, 85(10), pp. 1891-1901. ISSN 0887-3585. eISSN 1097-0134. Available under: doi: 10.1002/prot.25343

Zusammenfassung

The strawberry fruit allergens Fra a 1.01E, Fra a 1.02 and Fra a 1.03 belong to the group of pathogenesis-related 10 (PR-10) proteins and are homologs of the major birch pollen Bet v 1 and apple allergen Mal d 1. Bet v 1 related proteins are the most extensively studied allergens but their physiological function in planta remains elusive. Since Mal d 1-Associated Protein has been previously identified as interaction partner of Mal d 1 we studied the binding of the orthologous Fra a 1-Associated Protein (FaAP) to Fra a 1.01E/1.02/1.03. As the C-terminal sequence of FaAP showed strong auto-activation activity in yeast 2-hybrid analysis a novel time resolved DNA-switching system was successfully applied. Fra a 1.01E, Fra a 1.02, and Fra a 1.03 bind to FaAP with KD of 4.5 ± 1.1, 15 ± 3, and 11 ± 2 nM, respectively. Fra a 1.01E forms a dimer, whereas Fra a 1.02 and Fra a 1.03 bind as monomer. The results imply that PR-10 proteins might be integrated into a protein-interaction network and FaAP binding appears to be essential for the physiological function of the Fra a 1 proteins.

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ISO 690FRANZ-OBERDORF, Katrin, Andreas LANGER, Ralf STRASSER, Erika ISONO, Quirin L. RANFTL, Christian WUNSCHEL, Wilfried SCHWAB, 2017. Physical interaction between the strawberry allergen Fra a 1 and an associated partner FaAP : Interaction of Fra a 1 proteins and FaAP. In: Proteins : Structure, Function, and Bioinformatics. 2017, 85(10), pp. 1891-1901. ISSN 0887-3585. eISSN 1097-0134. Available under: doi: 10.1002/prot.25343
BibTex
@article{FranzOberdorf2017-10Physi-39858,
  year={2017},
  doi={10.1002/prot.25343},
  title={Physical interaction between the strawberry allergen Fra a 1 and an associated partner FaAP : Interaction of Fra a 1 proteins and FaAP},
  number={10},
  volume={85},
  issn={0887-3585},
  journal={Proteins : Structure, Function, and Bioinformatics},
  pages={1891--1901},
  author={Franz-Oberdorf, Katrin and Langer, Andreas and Strasser, Ralf and Isono, Erika and Ranftl, Quirin L. and Wunschel, Christian and Schwab, Wilfried}
}
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