Publikation: Influence of sodium concentration on changes of membrane capacitance associated with the electrogenic ion transport by the Na,K-ATPase
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Electrogenic ion transport by the Na,K-ATPase was investigated in a model system of protein-containing membrane fragments adsorbed to a lipid bilayer. Transient Na+ currents were induced by photorelease of ATP from inactive caged ATP. This process was accompanied by a capacitance change of the membrane system. Two methods were applied to measure capacitances in the frequency range 1 to 6000 Hz. It was, however, dependent on Na+ concentration and on the membrane potential which was generated by the charge transferred. Below 5 mM Na+ a negative capacitance change was detected which may be assigned to electrogenic Na+ binding to cytoplasmic sites. It could be shown that the results obtained by experiments with the presented alternating current method contain the information which is determined by current-relaxation experiments with cell membranes.
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SOKOLOV, Valerij S., S. M. STUKOLOV, A. S. DARMOSTUK, Hans-Jürgen APELL, 1998. Influence of sodium concentration on changes of membrane capacitance associated with the electrogenic ion transport by the Na,K-ATPase. In: European Biophysics Journal. 1998, 27(6), pp. 605-617. ISSN 0175-7571. eISSN 1432-1017. Available under: doi: 10.1007/s002490050172BibTex
@article{Sokolov1998Influ-8473, year={1998}, doi={10.1007/s002490050172}, title={Influence of sodium concentration on changes of membrane capacitance associated with the electrogenic ion transport by the Na,K-ATPase}, number={6}, volume={27}, issn={0175-7571}, journal={European Biophysics Journal}, pages={605--617}, author={Sokolov, Valerij S. and Stukolov, S. M. and Darmostuk, A. S. and Apell, Hans-Jürgen} }
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