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Biochemical and genotyping analyses of camels (Camelus dromedaries) trypanosomiasis in North Africa

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2023

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Darwish, Ahmed M.
Sharaf, Abdoallah
Gaouar, Semir Bechir Suheil
Ali, Neama I.
El-Aziz, Tamer H. Abd
Abushady, Asmaa M.
Kaouadji, Zoubeyda
Othman, Othman E.
Obornik, Miroslav

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Core Facility der Universität Konstanz
Sequencing Analysis Core Facility

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Scientific Reports. Springer Science and Business Media LLC. 2023, 13(1), 7176. eISSN 2045-2322. Verfügbar unter: doi: 10.1038/s41598-023-34086-y

Zusammenfassung

Abstract Camels are considered an important food source in North Africa. Trypanosomiasis in camels is a life-threatening disease that causes severe economic losses in milk and meat production. Therefore, the objective of this study was to determine the trypanosome genotypes in the North African region. Trypanosome infection rates were determined by microscopic examination of blood smears and polymerase chain reaction (PCR). In addition, total antioxidant capacity (TAC), lipid peroxides (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were determined in erythrocyte lysate. Furthermore, 18S amplicon sequencing was used to barcode and characterizes the genetic diversity of trypanosome genotypes in camel blood. In addition to Trypanosoma , Babesia and Thelieria were also detected in the blood samples. PCR showed that the trypanosome infection rate was higher in Algerian samples (25.7%) than in Egyptian samples (7.2%). Parameters such as MDA, GSH, SOD and CAT had significantly increased in camels infected with trypanosomes compared to uninfected control animals, while TAC level was not significantly changed. The results of relative amplicon abundance showed that the range of trypanosome infection was higher in Egypt than in Algeria. Moreover, phylogenetic analysis showed that the Trypanosoma sequences of Egyptian and Algerian camels are related to Trypanosoma evansi . Unexpectedly, diversity within T. evansi was higher in Egyptian camels than in Algerian camels. We present here the first molecular report providing a picture of trypanosomiasis in camels, covering wide geographical areas in Egypt and Algeria.

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570 Biowissenschaften, Biologie

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ISO 690DARWISH, Ahmed M., Abdoallah SHARAF, Semir Bechir Suheil GAOUAR, Neama I. ALI, Tamer H. Abd EL-AZIZ, Asmaa M. ABUSHADY, Zoubeyda KAOUADJI, Othman E. OTHMAN, Miroslav OBORNIK, 2023. Biochemical and genotyping analyses of camels (Camelus dromedaries) trypanosomiasis in North Africa. In: Scientific Reports. Springer Science and Business Media LLC. 2023, 13(1), 7176. eISSN 2045-2322. Verfügbar unter: doi: 10.1038/s41598-023-34086-y
BibTex
@article{Darwish2023-05-03Bioch-69003,
  year={2023},
  doi={10.1038/s41598-023-34086-y},
  title={Biochemical and genotyping analyses of camels (Camelus dromedaries) trypanosomiasis in North Africa},
  number={1},
  volume={13},
  journal={Scientific Reports},
  author={Darwish, Ahmed M. and Sharaf, Abdoallah and Gaouar, Semir Bechir Suheil and Ali, Neama I. and El-Aziz, Tamer H. Abd and Abushady, Asmaa M. and Kaouadji, Zoubeyda and Othman, Othman E. and Obornik, Miroslav},
  note={Article Number: 7176}
}
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