Publikation:

Topography for Independent Binding of α-Helical and PPII-Helical Ligands to a Peroxisomal SH3 Domain

Lade...
Vorschaubild

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2002

Autor:innen

Douangamath, Alice
Filipp, Fabian V
Klein, André T.J
Barnett, Phil
Zou, Peijian
Voorn-Brouwer, Tineke
Vega, M.Cristina
Sattler, Michael
Distel, Ben
et al.

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Molecular Cell. 2002, 10(5), pp. 1007-1017. ISSN 1097-2765. eISSN 1097-4164. Available under: doi: 10.1016/S1097-2765(02)00749-9

Zusammenfassung

While the function of most small signaling domains is confined to binary ligand interactions, the peroxisomal Pex13p SH3 domain has the unique capacity of binding to two different ligands, Pex5p and Pex14p. We have used this domain as a model to decipher its structurally independent ligand binding sites. By the combined use of X-ray crystallography, NMR spectroscopy, and circular dichroism, we show that the two ligands bind in unrelated conformations to patches located at opposite surfaces of this SH3 domain. Mutations in the Pex13p SH3 domain that abolish interactions within the Pex13p-Pex5p interface specifically impair PTS1-dependent protein import into yeast peroxisomes.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690DOUANGAMATH, Alice, Fabian V FILIPP, André T.J KLEIN, Phil BARNETT, Peijian ZOU, Tineke VOORN-BROUWER, M.Cristina VEGA, Olga MAYANS, Michael SATTLER, Ben DISTEL, 2002. Topography for Independent Binding of α-Helical and PPII-Helical Ligands to a Peroxisomal SH3 Domain. In: Molecular Cell. 2002, 10(5), pp. 1007-1017. ISSN 1097-2765. eISSN 1097-4164. Available under: doi: 10.1016/S1097-2765(02)00749-9
BibTex
@article{Douangamath2002Topog-42089,
  year={2002},
  doi={10.1016/S1097-2765(02)00749-9},
  title={Topography for Independent Binding of α-Helical and PPII-Helical Ligands to a Peroxisomal SH3 Domain},
  number={5},
  volume={10},
  issn={1097-2765},
  journal={Molecular Cell},
  pages={1007--1017},
  author={Douangamath, Alice and Filipp, Fabian V and Klein, André T.J and Barnett, Phil and Zou, Peijian and Voorn-Brouwer, Tineke and Vega, M.Cristina and Mayans, Olga and Sattler, Michael and Distel, Ben}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/42089">
    <dc:contributor>Klein, André T.J</dc:contributor>
    <dc:creator>Filipp, Fabian V</dc:creator>
    <dc:creator>Vega, M.Cristina</dc:creator>
    <dc:contributor>Filipp, Fabian V</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/42089"/>
    <dc:contributor>Distel, Ben</dc:contributor>
    <dc:creator>Mayans, Olga</dc:creator>
    <dc:contributor>Barnett, Phil</dc:contributor>
    <dc:contributor>Douangamath, Alice</dc:contributor>
    <dc:contributor>Voorn-Brouwer, Tineke</dc:contributor>
    <dc:creator>Zou, Peijian</dc:creator>
    <dc:creator>Douangamath, Alice</dc:creator>
    <dc:contributor>Mayans, Olga</dc:contributor>
    <dc:language>eng</dc:language>
    <dc:creator>Sattler, Michael</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-04-18T10:19:28Z</dcterms:available>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-04-18T10:19:28Z</dc:date>
    <dc:contributor>Zou, Peijian</dc:contributor>
    <dc:creator>Klein, André T.J</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Sattler, Michael</dc:contributor>
    <dcterms:abstract xml:lang="eng">While the function of most small signaling domains is confined to binary ligand interactions, the peroxisomal Pex13p SH3 domain has the unique capacity of binding to two different ligands, Pex5p and Pex14p. We have used this domain as a model to decipher its structurally independent ligand binding sites. By the combined use of X-ray crystallography, NMR spectroscopy, and circular dichroism, we show that the two ligands bind in unrelated conformations to patches located at opposite surfaces of this SH3 domain. Mutations in the Pex13p SH3 domain that abolish interactions within the Pex13p-Pex5p interface specifically impair PTS1-dependent protein import into yeast peroxisomes.</dcterms:abstract>
    <dc:creator>Distel, Ben</dc:creator>
    <dc:creator>Barnett, Phil</dc:creator>
    <dcterms:issued>2002</dcterms:issued>
    <dc:creator>Voorn-Brouwer, Tineke</dc:creator>
    <dcterms:title>Topography for Independent Binding of α-Helical and PPII-Helical Ligands to a Peroxisomal SH3 Domain</dcterms:title>
    <dc:contributor>Vega, M.Cristina</dc:contributor>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen