Publikation: Net synthesis of acetate from CO2 by Eubacterium acidaminophilum through the glycine reductase pathway
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Eubacterium acidaminophilum combines the oxidation of amino acids such as alanine or valine with the reduction of glycine to acetate in a two-substrate fermentation (Stickland reaction). In the absence of glycine, dense cell suspensions oxidized alanine or valine only to a small extent, with limited production of hydrogen and acetate. Experiments with 14C-labeled carbonate revealed that acetate was formed under these conditions by net reduction of CO2/HCO ; 14C-labeled formate was formed as an intermediate. E. acidaminophilum did not grow with hydrogen plus CO2 ; dense cell suspensions under H2/CO2 produced only very small amounts (60.5 mM) of acetate. There was no activity of carbon monoxide dehydrogenase, indicating that the glycine pathway was used for acetate synthesis. The results are explained on the basis of biochemical and energetic considerations.
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SCHNEEBERGER, Anne, Jochen FRINGS, Bernhard SCHINK, 1999. Net synthesis of acetate from CO2 by Eubacterium acidaminophilum through the glycine reductase pathway. In: FEMS Microbiology Letters. 1999, 177(1), pp. 1-6. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.1999.tb13705.xBibTex
@article{Schneeberger1999synth-8772, year={1999}, doi={10.1111/j.1574-6968.1999.tb13705.x}, title={Net synthesis of acetate from CO2 by Eubacterium acidaminophilum through the glycine reductase pathway}, number={1}, volume={177}, issn={0378-1097}, journal={FEMS Microbiology Letters}, pages={1--6}, author={Schneeberger, Anne and Frings, Jochen and Schink, Bernhard} }
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