Publikation: Expression and subcellular localization of mouse 20S proteasome activator complex PA28
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We have cloned the mouse PA28 proteasome activator cDNAs. Northern blot demonstrates high PA28 mRNA levels in liver, kidney and lung. mRNA levels are low in thymus, spleen and brain. In contrast, PA28 protein levels vary little between these tissues. Immunocytological analysis and cell fractionation experiments demonstrate that both subunits are almost equally distributed between the cytoplasm and the nucleus. Interestingly, PA28alpha spares nucleoli, while PA28beta is strongly enhanced in the nucleolus. This indicates for the first time that the PA28alpha and PA28beta subunits may serve nuclear functions which may be different from and independent of each other.
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SOZA, Andrea, Christine KNUEHL, Marcus GRÖTTRUP, Peter HENKLEIN, Keiji TANAKA, Peter M. KLOETZEL, 1997. Expression and subcellular localization of mouse 20S proteasome activator complex PA28. In: FEBS Letters. 1997, 413(1), pp. 27-34. ISSN 0014-5793. eISSN 1873-3468. Available under: doi: 10.1016/S0014-5793(97)00864-8BibTex
@article{Soza1997Expre-22244, year={1997}, doi={10.1016/S0014-5793(97)00864-8}, title={Expression and subcellular localization of mouse 20S proteasome activator complex PA28}, number={1}, volume={413}, issn={0014-5793}, journal={FEBS Letters}, pages={27--34}, author={Soza, Andrea and Knuehl, Christine and Gröttrup, Marcus and Henklein, Peter and Tanaka, Keiji and Kloetzel, Peter M.} }
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