Publikation:

Hydrolase and Peroxidase Activity of Gold Nanocluster Composites with Lysozyme and Cross-Linked Lysozyme Crystals

Lade...
Vorschaubild

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2025

Autor:innen

Lautenbach, Vanessa
Onishchukov, Georgy
Walter, Johannes
Gavira, José Antonio

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Deutsche Forschungsgemeinschaft (DFG): 416229255

Projekt

Open Access-Veröffentlichung
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

ACS Applied Nano Materials. ACS Publications. 2025, 8(1), S. 90-99. ISSN 2574-0970. eISSN 2574-0970. Verfügbar unter: doi: 10.1021/acsanm.4c04696

Zusammenfassung

Lysozyme (Lyz) and cross-linked Lyz crystals (CLLC) are among the proteins and templates used in the bioinspired synthesis of subnanometer-sized gold nanoclusters (AuNCs) utilized in various biological applications as biosensors, bioimaging agents, and recyclable catalyst. While previous studies have explored the formation and stabilization of AuNCs in the presence of Lyz, little is known about the enzymatic activity of Lyz after AuNCs stabilization in both its free form (AuNC-Lyz) and within CLLC (AuNC-CLLC). In this study, we conducted a comprehensive analysis of AuNC-Lyz, including hydrodynamic characteristics, using multiwavelength fluorescence analytical ultracentrifugation and enzymatic activity assays. Results show that AuNC-Lyz retains bactericidal performance at pH 4.0 and exhibits enhanced hydrolytic activity compared with Lyz alone. Additionally, AuNC-Lyz showed peroxidase-like performance due to the catalytically active surface of AuNCs providing the bifunctional catalytic capabilities of AuNC-Lyz. In contrast, fluorescent-based hydrolytic activity assays of AuNC-CLLC revealed that morphological variations in CLLC affect the catalytic performance. The AuNC-CLLC enzyme activity depends on the size of the solvent channels and Lyz conformation within CLLC. As a consequence of the occluded solvent channels and limited breathing (in/out diffusion) of CLLC, AuNC-CLLC displayed a diminishing hydrolytic performance.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
540 Chemie

Schlagwörter

fluorescent gold nanoclusters, lysozyme, bactericidal activity, mimic peroxidase, cross-linked lysozyme crystals

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690AKYÜZ, Özlem, Manuel BRODHAG, Vanessa LAUTENBACH, Marina KRUMOVA, Georgy ONISHCHUKOV, Johannes WALTER, José Antonio GAVIRA, Helmut CÖLFEN, 2025. Hydrolase and Peroxidase Activity of Gold Nanocluster Composites with Lysozyme and Cross-Linked Lysozyme Crystals. In: ACS Applied Nano Materials. ACS Publications. 2025, 8(1), S. 90-99. ISSN 2574-0970. eISSN 2574-0970. Verfügbar unter: doi: 10.1021/acsanm.4c04696
BibTex
@article{Akyuz2025-01-10Hydro-71129,
  title={Hydrolase and Peroxidase Activity of Gold Nanocluster Composites with Lysozyme and Cross-Linked Lysozyme Crystals},
  year={2025},
  doi={10.1021/acsanm.4c04696},
  number={1},
  volume={8},
  issn={2574-0970},
  journal={ACS Applied Nano Materials},
  pages={90--99},
  author={Akyüz, Özlem and Brodhag, Manuel and Lautenbach, Vanessa and Krumova, Marina and Onishchukov, Georgy and Walter, Johannes and Gavira, José Antonio and Cölfen, Helmut}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/71129">
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Cölfen, Helmut</dc:creator>
    <dc:creator>Akyüz, Özlem</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/71129"/>
    <dcterms:title>Hydrolase and Peroxidase Activity of Gold Nanocluster Composites with Lysozyme and Cross-Linked Lysozyme Crystals</dcterms:title>
    <dc:creator>Krumova, Marina</dc:creator>
    <dc:creator>Gavira, José Antonio</dc:creator>
    <dc:creator>Brodhag, Manuel</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:issued>2025-01-10</dcterms:issued>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2024-11-07T11:52:24Z</dc:date>
    <dc:contributor>Onishchukov, Georgy</dc:contributor>
    <dc:contributor>Cölfen, Helmut</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Krumova, Marina</dc:contributor>
    <dcterms:abstract>Lysozyme (Lyz) and cross-linked Lyz crystals (CLLC) are among the proteins and templates used in the bioinspired synthesis of subnanometer-sized gold nanoclusters (AuNCs) utilized in various biological applications as biosensors, bioimaging agents, and recyclable catalyst. While previous studies have explored the formation and stabilization of AuNCs in the presence of Lyz, little is known about the enzymatic activity of Lyz after AuNCs stabilization in both its free form (AuNC-Lyz) and within CLLC (AuNC-CLLC). In this study, we conducted a comprehensive analysis of AuNC-Lyz, including hydrodynamic characteristics, using multiwavelength fluorescence analytical ultracentrifugation and enzymatic activity assays. Results show that AuNC-Lyz retains bactericidal performance at pH 4.0 and exhibits enhanced hydrolytic activity compared with Lyz alone. Additionally, AuNC-Lyz showed peroxidase-like performance due to the catalytically active surface of AuNCs providing the bifunctional catalytic capabilities of AuNC-Lyz. In contrast, fluorescent-based hydrolytic activity assays of AuNC-CLLC revealed that morphological variations in CLLC affect the catalytic performance. The AuNC-CLLC enzyme activity depends on the size of the solvent channels and Lyz conformation within CLLC. As a consequence of the occluded solvent channels and limited breathing (in/out diffusion) of CLLC, AuNC-CLLC displayed a diminishing hydrolytic performance.</dcterms:abstract>
    <dc:creator>Walter, Johannes</dc:creator>
    <dc:creator>Lautenbach, Vanessa</dc:creator>
    <dc:creator>Onishchukov, Georgy</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2024-11-07T11:52:24Z</dcterms:available>
    <dc:contributor>Brodhag, Manuel</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:contributor>Lautenbach, Vanessa</dc:contributor>
    <dc:contributor>Gavira, José Antonio</dc:contributor>
    <dc:contributor>Walter, Johannes</dc:contributor>
    <dc:contributor>Akyüz, Özlem</dc:contributor>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
Diese Publikation teilen