Publikation: Use of chromosome microdissection in fish molecular cytogenetics
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2008
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Genetics and molecular biology. 2008, 31(1 suppl), pp. 279-283. Available under: doi: 10.1590/S1415-47572008000200022
Zusammenfassung
Chromosome microdissection is a technique in which whole chromosomes or chromosomal segments are dissected under an inverted microscope yielding chromosome-specific sequences. Several protocol modifications introduced during the past 15 years reduced the number of chromosomes required for most applications. This is of particular interest to fish molecular cytogenetics, since most species present highly uniform karyotypes which make impossible the collection of multiple copies of the same chromosome. Probes developed in this manner can be used to investigate chromosome homologies in closely related species. Here we describe a protocol recently used in the gymnotiform species group Eigenmannia and review the major steps involved in the generation of these markers focusing on protocol modifications aiming to reduce the number of required chromosomes.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
fluorescence in situ hybridization, chromosome painting, sex chromosomes, cross-FISH
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HENNING, Frederico, Vladimir TRIFONOV, Lurdes Foresti de ALMEIDA-TOLEDO, 2008. Use of chromosome microdissection in fish molecular cytogenetics. In: Genetics and molecular biology. 2008, 31(1 suppl), pp. 279-283. Available under: doi: 10.1590/S1415-47572008000200022BibTex
@article{Henning2008chrom-8007,
year={2008},
doi={10.1590/S1415-47572008000200022 },
title={Use of chromosome microdissection in fish molecular cytogenetics},
number={1 suppl},
volume={31},
journal={Genetics and molecular biology},
pages={279--283},
author={Henning, Frederico and Trifonov, Vladimir and Almeida-Toledo, Lurdes Foresti de}
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