Publikation:

Highly standardized multicolor femtosecond fiber system for selective microphotomanipulation of deoxyribonucleic acid and chromatin

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2018

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Schindler, Felix

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http://nbn-resolving.de/urn:nbn:de:bsz:352-2-15ij7j0tgdvgz6
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https://doi.org/10.1364/OL.43.002877
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Optics Letters. 2018, 43(12), pp. 2877-2880. ISSN 0146-9592. eISSN 1539-4794. Available under: doi: 10.1364/OL.43.002877

Zusammenfassung

We present a three-color femtosecond Er/Yb:fiber laser enabling highly specific and standardized nonlinear optical manipulation of live cells. The system simultaneously provides bandwidth-limited 80-fs pulses with identical intensity envelope centered at wavelengths of 515, 775, and 1035 nm in the focus of a confocal microscope. We achieve this goal by combining high-order dispersion control via, for example, chirped fiber Bragg gratings with proper bandwidth management in each nonlinear conversion step. Wavelength-selective and noninterfering induction of deoxyribonucleic acid (DNA) photoproducts and DNA strand breaks, as well as fluorescence photoactivation of a photoactivatable green fluorescent protein (PA-GFP)–histone fusion protein, are demonstrated. The capability to introduce different types of DNA lesions and perform photoswitching experiments in a selective manner is essential for quantitative studies on DNA repair and chromatin dynamics.

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530 Physik

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ISO 690SCHMALZ, Michael, Ines WIESER, Felix SCHINDLER, Christina CZADA, Alfred LEITENSTORFER, Elisa FERRANDO-MAY, 2018. Highly standardized multicolor femtosecond fiber system for selective microphotomanipulation of deoxyribonucleic acid and chromatin. In: Optics Letters. 2018, 43(12), pp. 2877-2880. ISSN 0146-9592. eISSN 1539-4794. Available under: doi: 10.1364/OL.43.002877
BibTex
@article{Schmalz2018-06-15Highl-42850,
  year={2018},
  doi={10.1364/OL.43.002877},
  title={Highly standardized multicolor femtosecond fiber system for selective microphotomanipulation of deoxyribonucleic acid and chromatin},
  number={12},
  volume={43},
  issn={0146-9592},
  journal={Optics Letters},
  pages={2877--2880},
  author={Schmalz, Michael and Wieser, Ines and Schindler, Felix and Czada, Christina and Leitenstorfer, Alfred and Ferrando-May, Elisa}
}
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Interner Vermerk

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