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Metabolic pathways and energetics of the acetone-oxidizing, sulfate-reducing bacterium, Desulfobacterium cetonicum

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1995

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Janssen, Peter H.

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Archives of Microbiology. 1995, 163(3), pp. 188-194. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00305352

Zusammenfassung

Acetone degradation by cell suspensions of Desulfobacterium cetonicum was CO2-dependent, indicating initiation by a carboxylation reaction. Degradation of butyrate was not CO2-dependent, and acetate accumulated at a ratio of 1 mol acetate per mol butyrate degraded. In cultures grown on acetone, no CoA transfer apparently occurred, and no acetate accumulated in the medium. No CoA-ligase activities were detected in cell-free crude extracts. This suggested that the carboxylation of acetone to acetoacetate; and its activation to acetoacetyl-CoA may occur without the formation of free acetoacetate. Acetoacetyl-CoA was thiolytically cleaved to two acetyl-CoA, which were oxidized to CO2 via the acetyl-CoA/carbon monoxide dehydrogenase pathway. The measured intracellular acyl-CoA ester concentrations allowed the calculation of the free energy changes involved in the conversion of acetone to acetyl-CoA. At in vivo concentrations of reactants and products, the initial steps (carboxylation and activation) must be energy-driven, either by direct coupling to ATE or coupling to transmembrane gradients. The AG' of acetone conversion to two acetyl-CoA at the expense of the energetic equivalent of one ATP was calculated to lie very close to 0kJ (mol acetone) ~. Assimilatory metabolism was by an incomplete citric acid cycle, lacking an activity oxidatively decarboxylating 2-oxoglutarate. The low specific activities of this cycle suggested its probable function in anabolic metabolism. Succinate and glyoxylate were formed from isocitrate by isocitrate lyase. Glyoxylate thus formed was condensed with acetyl-CoA to form malate, functioning as an anaplerotic sequence. A glyoxylate cycle thus operates in this strictly anaerobic bacterium. Phosphoenolpyruvate (PEP) carboxykinase formed PEP from oxaloacetate. No pyruvate kinase activity was detected. PEP presumably served as a precursor for polyglucose formation and other biosyntheses.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie

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Anaerobic degradation, Acetone, Carboxylation, Energetics, Sulfate-reducing bacterium, Desulfobacterium cetonicurn, Citric acid cycle

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ISO 690JANSSEN, Peter H., Bernhard SCHINK, 1995. Metabolic pathways and energetics of the acetone-oxidizing, sulfate-reducing bacterium, Desulfobacterium cetonicum. In: Archives of Microbiology. 1995, 163(3), pp. 188-194. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00305352
BibTex
@article{Janssen1995Metab-6539,
  year={1995},
  doi={10.1007/BF00305352},
  title={Metabolic pathways and energetics of the acetone-oxidizing, sulfate-reducing bacterium, Desulfobacterium cetonicum},
  number={3},
  volume={163},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={188--194},
  author={Janssen, Peter H. and Schink, Bernhard}
}
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