Publikation:

Small-angle scattering as a tool to study the thermal denaturation of DNA

Vorschaubild

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2014

Autor:innen

Wood, Kathleen
Knott, Robert
Tonchev, Ognyan
Angelov, Dimitar
Peyrard, Michel

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
DOI (zitierfähiger Link)
https://doi.org/10.1209/0295-5075/108/18002
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung

Sammlungen

Physik: Publikationen
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

EPL : Europhysics Letters. 2014, 108(1), 18002. ISSN 0295-5075. eISSN 1286-4854. Available under: doi: 10.1209/0295-5075/108/18002

Zusammenfassung

DNA thermal denaturation is the breaking of the base pairs, leading to a splitting of the two strands of the double helix. While it is easy to measure the fraction of open base pairs (f) vs. temperature, determining the fraction (p) of fully open molecules is much harder. Previously, the simultaneous recording of f and p could only be achieved for special sequences. We show that small-angle scattering of X-rays or neutrons allows the measurement of p for any sequence. We illustrate the method with a SAXS investigation of two sequences designed to exhibit different melting profiles and compare the SAXS data with nano-calorimetric measurements of the melting curve.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
530 Physik

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690WOOD, Kathleen, Robert KNOTT, Ognyan TONCHEV, Dimitar ANGELOV, Nikos THEODORAKOPOULOS, Michel PEYRARD, 2014. Small-angle scattering as a tool to study the thermal denaturation of DNA. In: EPL : Europhysics Letters. 2014, 108(1), 18002. ISSN 0295-5075. eISSN 1286-4854. Available under: doi: 10.1209/0295-5075/108/18002
BibTex
@article{Wood2014Small-30027,
  year={2014},
  doi={10.1209/0295-5075/108/18002},
  title={Small-angle scattering as a tool to study the thermal denaturation of DNA},
  number={1},
  volume={108},
  issn={0295-5075},
  journal={EPL : Europhysics Letters},
  author={Wood, Kathleen and Knott, Robert and Tonchev, Ognyan and Angelov, Dimitar and Theodorakopoulos, Nikos and Peyrard, Michel},
  note={Article Number: 18002}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/30027">
    <dcterms:abstract xml:lang="eng">DNA thermal denaturation is the breaking of the base pairs, leading to a splitting of the two strands of the double helix. While it is easy to measure the fraction of open base pairs (f) vs. temperature, determining the fraction (p) of fully open molecules is much harder. Previously, the simultaneous recording of f and p could only be achieved for special sequences. We show that small-angle scattering of X-rays or neutrons allows the measurement of p for any sequence. We illustrate the method with a SAXS investigation of two sequences designed to exhibit different melting profiles and compare the SAXS data with nano-calorimetric measurements of the melting curve.</dcterms:abstract>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/41"/>
    <dc:creator>Angelov, Dimitar</dc:creator>
    <dc:contributor>Theodorakopoulos, Nikos</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-02-25T09:07:38Z</dc:date>
    <dc:creator>Wood, Kathleen</dc:creator>
    <dcterms:title>Small-angle scattering as a tool to study the thermal denaturation of DNA</dcterms:title>
    <dc:contributor>Wood, Kathleen</dc:contributor>
    <dc:contributor>Peyrard, Michel</dc:contributor>
    <dc:language>eng</dc:language>
    <dc:contributor>Tonchev, Ognyan</dc:contributor>
    <dc:creator>Theodorakopoulos, Nikos</dc:creator>
    <dc:creator>Knott, Robert</dc:creator>
    <dc:contributor>Knott, Robert</dc:contributor>
    <dc:contributor>Angelov, Dimitar</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/30027"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-02-25T09:07:38Z</dcterms:available>
    <dc:creator>Peyrard, Michel</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/41"/>
    <dc:creator>Tonchev, Ognyan</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:issued>2014</dcterms:issued>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen