Publikation:

Arabidopsis CaLB1 undergoes phase separation with the ESCRT protein ALIX and modulates autophagosome maturation

Lade...
Vorschaubild

Dateien

Mosesso_2-13pvhb93n51f06.pdf
Mosesso_2-13pvhb93n51f06.pdfGröße: 4.91 MBDownloads: 12

Datum

2024

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Link zur Lizenz

Angaben zur Forschungsförderung

Deutsche Forschungsgemeinschaft (DFG): 189682160
Deutsche Forschungsgemeinschaft (DFG): SFB969
Deutsche Forschungsgemeinschaft (DFG): INST 38/579-1
Deutsche Forschungsgemeinschaft (DFG): 496470458

Projekt

Open Access-Veröffentlichung
Open Access Gold
Core Facility der Universität Konstanz
Bioimaging Centre, Electron Microscopy Centre

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Nature Communications. Springer. 2024, 15(1), 5188. eISSN 2041-1723. Verfügbar unter: doi: 10.1038/s41467-024-49485-6

Zusammenfassung

Autophagy is relevant for diverse processes in eukaryotic cells, making its regulation of fundamental importance. The formation and maturation of autophagosomes require a complex choreography of numerous factors. The endosomal sorting complex required for transport (ESCRT) is implicated in the final step of autophagosomal maturation by sealing of the phagophore membrane. ESCRT-III components were shown to mediate membrane scission by forming filaments that interact with cellular membranes. However, the molecular mechanisms underlying the recruitment of ESCRTs to non-endosomal membranes remain largely unknown. Here we focus on the ESCRT-associated protein ALG2-interacting protein X (ALIX) and identify Ca 2+ -dependent lipid binding protein 1 (CaLB1) as its interactor. Our findings demonstrate that CaLB1 interacts with AUTOPHAGY8 (ATG8) and PI(3)P, a phospholipid found in autophagosomal membranes. Moreover, CaLB1 and ALIX localize with ATG8 on autophagosomes upon salt treatment and assemble together into condensates. The depletion of CaLB1 impacts the maturation of salt-induced autophagosomes and leads to reduced delivery of autophagosomes to the vacuole. Here, we propose a crucial role of CaLB1 in augmenting phase separation of ALIX, facilitating the recruitment of ESCRT-III to the site of phagophore closure thereby ensuring efficient maturation of autophagosomes.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
540 Chemie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690MOSESSO, Niccolo, Niharika Savant LERNER, Tobias BLÄSKE, Felix GROH, Shane MAGUIRE, Marie L. NIEDERMEIER, Eliane LANDWEHR, Karin VOGEL, Konstanze MEERGANS, Marie-Kristin NAGEL, Malte DRESCHER, Florian STENGEL, Karin HAUSER, Erika ISONO, 2024. Arabidopsis CaLB1 undergoes phase separation with the ESCRT protein ALIX and modulates autophagosome maturation. In: Nature Communications. Springer. 2024, 15(1), 5188. eISSN 2041-1723. Verfügbar unter: doi: 10.1038/s41467-024-49485-6
BibTex
@article{Mosesso2024-06-19Arabi-70551,
  year={2024},
  doi={10.1038/s41467-024-49485-6},
  title={Arabidopsis CaLB1 undergoes phase separation with the ESCRT protein ALIX and modulates autophagosome maturation},
  number={1},
  volume={15},
  journal={Nature Communications},
  author={Mosesso, Niccolo and Lerner, Niharika Savant and Bläske, Tobias and Groh, Felix and Maguire, Shane and Niedermeier, Marie L. and Landwehr, Eliane and Vogel, Karin and Meergans, Konstanze and Nagel, Marie-Kristin and Drescher, Malte and Stengel, Florian and Hauser, Karin and Isono, Erika},
  note={Article Number: 5188}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/70551">
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dc:contributor>Drescher, Malte</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Maguire, Shane</dc:contributor>
    <dcterms:title>Arabidopsis CaLB1 undergoes phase separation with the ESCRT protein ALIX and modulates autophagosome maturation</dcterms:title>
    <dc:contributor>Landwehr, Eliane</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/70551"/>
    <dc:contributor>Nagel, Marie-Kristin</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Stengel, Florian</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/70551/1/Mosesso_2-13pvhb93n51f06.pdf"/>
    <dc:contributor>Vogel, Karin</dc:contributor>
    <dc:contributor>Groh, Felix</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Nagel, Marie-Kristin</dc:creator>
    <dcterms:abstract>Autophagy is relevant for diverse processes in eukaryotic cells, making its regulation of fundamental importance. The formation and maturation of autophagosomes require a complex choreography of numerous factors. The endosomal sorting complex required for transport (ESCRT) is implicated in the final step of autophagosomal maturation by sealing of the phagophore membrane. ESCRT-III components were shown to mediate membrane scission by forming filaments that interact with cellular membranes. However, the molecular mechanisms underlying the recruitment of ESCRTs to non-endosomal membranes remain largely unknown. Here we focus on the ESCRT-associated protein ALG2-interacting protein X (ALIX) and identify Ca &lt;sup&gt;2+&lt;/sup&gt; -dependent lipid binding protein 1 (CaLB1) as its interactor. Our findings demonstrate that CaLB1 interacts with AUTOPHAGY8 (ATG8) and PI(3)P, a phospholipid found in autophagosomal membranes. Moreover, CaLB1 and ALIX localize with ATG8 on autophagosomes upon salt treatment and assemble together into condensates. The depletion of CaLB1 impacts the maturation of salt-induced autophagosomes and leads to reduced delivery of autophagosomes to the vacuole. Here, we propose a crucial role of CaLB1 in augmenting phase separation of ALIX, facilitating the recruitment of ESCRT-III to the site of phagophore closure thereby ensuring efficient maturation of autophagosomes.</dcterms:abstract>
    <dc:creator>Drescher, Malte</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:issued>2024-06-19</dcterms:issued>
    <dc:creator>Mosesso, Niccolo</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Landwehr, Eliane</dc:creator>
    <dc:creator>Lerner, Niharika Savant</dc:creator>
    <dc:contributor>Meergans, Konstanze</dc:contributor>
    <dc:contributor>Niedermeier, Marie L.</dc:contributor>
    <dc:contributor>Bläske, Tobias</dc:contributor>
    <dc:creator>Bläske, Tobias</dc:creator>
    <dc:contributor>Hauser, Karin</dc:contributor>
    <dc:creator>Stengel, Florian</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/70551/1/Mosesso_2-13pvhb93n51f06.pdf"/>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dc:creator>Maguire, Shane</dc:creator>
    <dc:contributor>Lerner, Niharika Savant</dc:contributor>
    <dc:creator>Niedermeier, Marie L.</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Meergans, Konstanze</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2024-08-07T10:48:31Z</dcterms:available>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2024-08-07T10:48:31Z</dc:date>
    <dc:creator>Vogel, Karin</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Isono, Erika</dc:contributor>
    <dc:creator>Hauser, Karin</dc:creator>
    <dc:creator>Isono, Erika</dc:creator>
    <dc:creator>Groh, Felix</dc:creator>
    <dc:contributor>Mosesso, Niccolo</dc:contributor>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
Diese Publikation teilen