Publikation: Modified Oligonucleotides as Tools for Allele-Specific Amplification
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2007
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YURYEV, Anton, ed.. PCR Primer Design. Totowa, NJ: Humana Press, 2007, pp. 317-328. Methods in Molecular Biology. 402. ISBN 978-1-58829-725-9. Available under: doi: 10.1007/978-1-59745-528-2_16
Zusammenfassung
Allele-specific polymerase chain reaction (PCR), a method that reports nucleotide variations through either the presence or the absence of a DNA product obtained through PCR amplification, holds the promise to combine target amplification and analysis in one single step. Recently, it has been reported that the selectivity of allele-specific PCR can be significantly increased through the employment of chemically modified primer probes. Here, we report on significant developments of primer probe design and synthesis along this line.
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STRERATH, Michael, Ilka DETMER, Jens GASTER, Andreas MARX, 2007. Modified Oligonucleotides as Tools for Allele-Specific Amplification. In: YURYEV, Anton, ed.. PCR Primer Design. Totowa, NJ: Humana Press, 2007, pp. 317-328. Methods in Molecular Biology. 402. ISBN 978-1-58829-725-9. Available under: doi: 10.1007/978-1-59745-528-2_16BibTex
@incollection{Strerath2007Modif-33928,
year={2007},
doi={10.1007/978-1-59745-528-2_16},
title={Modified Oligonucleotides as Tools for Allele-Specific Amplification},
number={402},
isbn={978-1-58829-725-9},
publisher={Humana Press},
address={Totowa, NJ},
series={Methods in Molecular Biology},
booktitle={PCR Primer Design},
pages={317--328},
editor={Yuryev, Anton},
author={Strerath, Michael and Detmer, Ilka and Gaster, Jens and Marx, Andreas}
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<dcterms:abstract xml:lang="eng">Allele-specific polymerase chain reaction (PCR), a method that reports nucleotide variations through either the presence or the absence of a DNA product obtained through PCR amplification, holds the promise to combine target amplification and analysis in one single step. Recently, it has been reported that the selectivity of allele-specific PCR can be significantly increased through the employment of chemically modified primer probes. Here, we report on significant developments of primer probe design and synthesis along this line.</dcterms:abstract>
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