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Probing the Structural Dynamics of a Bacterial Chaperone in Its Native Environment by Nitroxide-Based EPR Spectroscopy

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2022

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Bonucci, Alessio
Normanno, Davide
Ansaldi, Mireille
Pilet, Eric
Ouari, Olivier
Guigliarelli, Bruno
Etienne, Emilien
Gerbaud, Guillaume
Mileo, Elisabetta
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Chemistry - A European Journal. Wiley. 2022, 28(66), e202202249. ISSN 0947-6539. eISSN 1521-3765. Verfügbar unter: doi: 10.1002/chem.202202249

Zusammenfassung

One of the greatest current challenges in structural biology is to study protein dynamics over a wide range of timescales in complex environments, such as the cell. Among magnetic resonances suitable for this approach, electron paramagnetic resonance spectroscopy coupled to site-directed spin labeling (SDSL-EPR) has emerged as a promising tool to study protein local dynamics and conformational ensembles. In this work, we exploit the sensitivity of nitroxide labels to report protein local dynamics at room temperature. We demonstrate that such studies can be performed while preserving both the integrity of the cells and the activity of the protein under investigation. Using this approach, we studied the structural dynamics of the chaperone NarJ in its natural host, Escherichia coli. We established that spin-labeled NarJ is active inside the cell. We showed that the cellular medium affects NarJ structural dynamics in a site-specific way, while the structural flexibility of the protein is maintained. Finally, we present and discuss data on the time-resolved dynamics of NarJ in cellular context.

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540 Chemie

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ISO 690PIERRO, Annalisa, Alessio BONUCCI, Davide NORMANNO, Mireille ANSALDI, Eric PILET, Olivier OUARI, Bruno GUIGLIARELLI, Emilien ETIENNE, Guillaume GERBAUD, Elisabetta MILEO, 2022. Probing the Structural Dynamics of a Bacterial Chaperone in Its Native Environment by Nitroxide-Based EPR Spectroscopy. In: Chemistry - A European Journal. Wiley. 2022, 28(66), e202202249. ISSN 0947-6539. eISSN 1521-3765. Verfügbar unter: doi: 10.1002/chem.202202249
BibTex
@article{Pierro2022-11-25Probi-59184,
  year={2022},
  doi={10.1002/chem.202202249},
  title={Probing the Structural Dynamics of a Bacterial Chaperone in Its Native Environment by Nitroxide-Based EPR Spectroscopy},
  number={66},
  volume={28},
  issn={0947-6539},
  journal={Chemistry - A European Journal},
  author={Pierro, Annalisa and Bonucci, Alessio and Normanno, Davide and Ansaldi, Mireille and Pilet, Eric and Ouari, Olivier and Guigliarelli, Bruno and Etienne, Emilien and Gerbaud, Guillaume and Mileo, Elisabetta},
  note={Article Number: e202202249}
}
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