Publikation: Probing the Structural Dynamics of a Bacterial Chaperone in Its Native Environment by Nitroxide-Based EPR Spectroscopy
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
One of the greatest current challenges in structural biology is to study protein dynamics over a wide range of timescales in complex environments, such as the cell. Among magnetic resonances suitable for this approach, electron paramagnetic resonance spectroscopy coupled to site-directed spin labeling (SDSL-EPR) has emerged as a promising tool to study protein local dynamics and conformational ensembles. In this work, we exploit the sensitivity of nitroxide labels to report protein local dynamics at room temperature. We demonstrate that such studies can be performed while preserving both the integrity of the cells and the activity of the protein under investigation. Using this approach, we studied the structural dynamics of the chaperone NarJ in its natural host, Escherichia coli. We established that spin-labeled NarJ is active inside the cell. We showed that the cellular medium affects NarJ structural dynamics in a site-specific way, while the structural flexibility of the protein is maintained. Finally, we present and discuss data on the time-resolved dynamics of NarJ in cellular context.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
PIERRO, Annalisa, Alessio BONUCCI, Davide NORMANNO, Mireille ANSALDI, Eric PILET, Olivier OUARI, Bruno GUIGLIARELLI, Emilien ETIENNE, Guillaume GERBAUD, Elisabetta MILEO, 2022. Probing the Structural Dynamics of a Bacterial Chaperone in Its Native Environment by Nitroxide-Based EPR Spectroscopy. In: Chemistry - A European Journal. Wiley. 2022, 28(66), e202202249. ISSN 0947-6539. eISSN 1521-3765. Verfügbar unter: doi: 10.1002/chem.202202249BibTex
@article{Pierro2022-11-25Probi-59184, year={2022}, doi={10.1002/chem.202202249}, title={Probing the Structural Dynamics of a Bacterial Chaperone in Its Native Environment by Nitroxide-Based EPR Spectroscopy}, number={66}, volume={28}, issn={0947-6539}, journal={Chemistry - A European Journal}, author={Pierro, Annalisa and Bonucci, Alessio and Normanno, Davide and Ansaldi, Mireille and Pilet, Eric and Ouari, Olivier and Guigliarelli, Bruno and Etienne, Emilien and Gerbaud, Guillaume and Mileo, Elisabetta}, note={Article Number: e202202249} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/59184"> <dc:contributor>Pierro, Annalisa</dc:contributor> <dcterms:title>Probing the Structural Dynamics of a Bacterial Chaperone in Its Native Environment by Nitroxide-Based EPR Spectroscopy</dcterms:title> <dc:creator>Guigliarelli, Bruno</dc:creator> <dc:contributor>Ansaldi, Mireille</dc:contributor> <dc:contributor>Pilet, Eric</dc:contributor> <dc:creator>Pilet, Eric</dc:creator> <dc:creator>Bonucci, Alessio</dc:creator> <dc:creator>Ansaldi, Mireille</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/59184/1/Pierro_2-12w9yp81ctxt76.PDF"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/> <dc:contributor>Mileo, Elisabetta</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Normanno, Davide</dc:creator> <dc:contributor>Bonucci, Alessio</dc:contributor> <dc:creator>Mileo, Elisabetta</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/59184"/> <dc:creator>Pierro, Annalisa</dc:creator> <dc:creator>Ouari, Olivier</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-11-18T08:23:43Z</dcterms:available> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/59184/1/Pierro_2-12w9yp81ctxt76.PDF"/> <dc:contributor>Ouari, Olivier</dc:contributor> <dcterms:abstract xml:lang="eng">One of the greatest current challenges in structural biology is to study protein dynamics over a wide range of timescales in complex environments, such as the cell. Among magnetic resonances suitable for this approach, electron paramagnetic resonance spectroscopy coupled to site-directed spin labeling (SDSL-EPR) has emerged as a promising tool to study protein local dynamics and conformational ensembles. In this work, we exploit the sensitivity of nitroxide labels to report protein local dynamics at room temperature. We demonstrate that such studies can be performed while preserving both the integrity of the cells and the activity of the protein under investigation. Using this approach, we studied the structural dynamics of the chaperone NarJ in its natural host, Escherichia coli. We established that spin-labeled NarJ is active inside the cell. We showed that the cellular medium affects NarJ structural dynamics in a site-specific way, while the structural flexibility of the protein is maintained. Finally, we present and discuss data on the time-resolved dynamics of NarJ in cellular context.</dcterms:abstract> <dc:contributor>Normanno, Davide</dc:contributor> <dc:creator>Etienne, Emilien</dc:creator> <dc:contributor>Guigliarelli, Bruno</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dc:contributor>Gerbaud, Guillaume</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/> <dc:contributor>Etienne, Emilien</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-11-18T08:23:43Z</dc:date> <dcterms:issued>2022-11-25</dcterms:issued> <dc:creator>Gerbaud, Guillaume</dc:creator> </rdf:Description> </rdf:RDF>