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T cell response to human papillomavirus 16 E7 in mice : comparison of Cr release assay, intracellular IFN-g=y [gamma] production, ELISPOT and tetramer staining

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2002

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Michel, Nico
Osen, Wolfram
Freyschmidt, Eva-Jasmin
Guthöhrlein, Heidrun
Kaufmann, Andreas M.
Müller, Martin
Gissmann, Lutz

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Intervirology. 2002, 45(4-6), pp. 290-299. ISSN 1423-0100. Available under: doi: 10.1159/000067923

Zusammenfassung

Successful vaccination against infections by high-risk papillomaviruses aiming at the prevention of cervical cancer most likely requires the induction of neutralizing antibodies and human papillomavirus (HPV)-specific T cells directed against early viral proteins such as E7. Whereas the technology for detection of antibodies is well established, measurement of T cells is more cumbersome and standardization of assays is difficult. By using chromium release assay, ELISPOT, tetramer staining and intracellular IFN-gamma assay, we compared the levels of HPV 16 E7-specific T cells obtained after immunization of C57BL/6 mice with different DNA expression vectors. We found that all four assays gave highly comparable results. ELISPOT can be recommended for future studies as it indicates the presence of activated (i.e. IFN-gamma-secreting) T cells in a quantitative manner and combines high sensitivity with relatively low T cell demand.

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570 Biowissenschaften, Biologie

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elispot, ifn-gamma, tetramer stain, intracellular ifn-gamma, papillomaviru, cell, tetramer, human papillomaviru, mice, release assay, hpv, assay intracellular, ifn-gamma production, assay, mice comparison

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ISO 690MICHEL, Nico, Peter ÖHLSCHLÄGER, Wolfram OSEN, Eva-Jasmin FREYSCHMIDT, Heidrun GUTHÖHRLEIN, Andreas M. KAUFMANN, Martin MÜLLER, Lutz GISSMANN, 2002. T cell response to human papillomavirus 16 E7 in mice : comparison of Cr release assay, intracellular IFN-g=y [gamma] production, ELISPOT and tetramer staining. In: Intervirology. 2002, 45(4-6), pp. 290-299. ISSN 1423-0100. Available under: doi: 10.1159/000067923
BibTex
@article{Michel2002respo-17505,
  year={2002},
  doi={10.1159/000067923},
  title={T cell response to human papillomavirus 16 E7 in mice : comparison of Cr release assay, intracellular IFN-g=y [gamma] production, ELISPOT and tetramer staining},
  number={4-6},
  volume={45},
  issn={1423-0100},
  journal={Intervirology},
  pages={290--299},
  author={Michel, Nico and Öhlschläger, Peter and Osen, Wolfram and Freyschmidt, Eva-Jasmin and Guthöhrlein, Heidrun and Kaufmann, Andreas M. and Müller, Martin and Gissmann, Lutz}
}
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