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Molecular Cloning of Two New Interferon-induced, Highly Related Nuclear Phosphoproteins

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Kadereit_et_al._1993.pdf
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1993

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Gewert, Dirk R.
Galabru, Julien
Hovanessian, Ara G.
Meurs, Eliane F.

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Journal of Biological Chemistry. 1993, 268(32), pp. 24432-24441

Zusammenfassung

During the molecular cloning of the human dsRNA activated-p68 kinase (PKR), polyclonal antibodies against PKR selected, in addition to cDNAs corresponding to PKR, another cDNA presenting only slight homology with PKR cDNA. This cDNA recognized an mRNA species of 2 kilobases induced by both α- and γ- interferons. Its transcription did not require protein synthesis. On further library screening, it selected two highly related cDNAs, referred to as 75 and 41, displaying perfect homology over 612 base pairs and divergent at both ends. In addition, cDNA 75 presents an insertion of 150 base pairs highly homologous to a region common to both sequences. The 75 and 41 peptidic sequences are very hydrophilic, rich in basic amino acid residues, and contain several potential phosphorylation sites for different serine/threonine kinases. Furthermore, they present two protamine and histone-like nuclear targeting sequencaess well as some homology with helix-loop-helix motifs of some DNA-binding proteins. The 75-encoded product, which resolved as a 52-kDa protein after in vitro expression in rabbit reticulocyte lysates, was found to migrate as a 65-67-kDa protein after in vivo expression in insect cells. In accord with sequence data, this 65-67-kDa protein was found to be phosphorylated in vivo in the insect cells and was recovered from the membrane/nuclear pellet. In contrast, the 41-encodedproduct (30-kDa protein in reticulocyte lysates) could not be expressed in vivo, as it provoked a rapid and severe shutoff of protein synthesis in insect cells. The function of the 75 and 41 proteins and their relation to PKR remains to be determined. However, the presence of nuclear targeting sequences, phosphorylation sites, and helix-loop-helix motif is consistent with a role of these proteins in the mechanism of transduction of the interferon action.

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570 Biowissenschaften, Biologie

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ISO 690KADEREIT, Suzanne, Dirk R. GEWERT, Julien GALABRU, Ara G. HOVANESSIAN, Eliane F. MEURS, 1993. Molecular Cloning of Two New Interferon-induced, Highly Related Nuclear Phosphoproteins. In: Journal of Biological Chemistry. 1993, 268(32), pp. 24432-24441
BibTex
@article{Kadereit1993Molec-6558,
  year={1993},
  title={Molecular Cloning of Two New Interferon-induced, Highly Related Nuclear Phosphoproteins},
  number={32},
  volume={268},
  journal={Journal of Biological Chemistry},
  pages={24432--24441},
  author={Kadereit, Suzanne and Gewert, Dirk R. and Galabru, Julien and Hovanessian, Ara G. and Meurs, Eliane F.}
}
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    <dcterms:abstract xml:lang="eng">During the molecular cloning of the human dsRNA activated-p68 kinase (PKR), polyclonal antibodies against PKR selected, in addition to cDNAs corresponding to PKR, another cDNA presenting only slight homology with PKR cDNA. This cDNA recognized an mRNA species of 2 kilobases induced by both α-  and γ- interferons. Its transcription did not require protein synthesis. On further library screening, it selected two highly related cDNAs, referred to as 75 and 41, displaying perfect homology over 612 base pairs and divergent at both ends. In addition, cDNA 75 presents an insertion of 150 base pairs highly homologous to a region common to both sequences. The 75 and 41 peptidic sequences are very hydrophilic, rich in basic amino acid residues, and contain several potential phosphorylation sites for different serine/threonine kinases. Furthermore, they present two protamine and histone-like nuclear targeting sequencaess well as some homology with helix-loop-helix motifs of some DNA-binding proteins. The 75-encoded product, which resolved as a 52-kDa protein after in vitro expression in rabbit reticulocyte lysates, was found to migrate as a 65-67-kDa protein after in vivo expression in insect cells. In accord with sequence data, this 65-67-kDa protein was found to be phosphorylated in vivo in the insect cells and was recovered from the membrane/nuclear pellet. In contrast, the 41-encodedproduct (30-kDa protein in reticulocyte lysates) could not be expressed in vivo, as it provoked a rapid and severe shutoff of protein synthesis in insect cells. The function of the 75 and 41 proteins and their relation to PKR remains to be determined. However, the presence of nuclear targeting sequences, phosphorylation sites, and helix-loop-helix motif is consistent with a role of these proteins in the mechanism of transduction of the interferon action.</dcterms:abstract>
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