Publikation: Using Systems Approaches to Analyze Metabolic Networks Involved in Storage Reserve Synthesis in Developing Seeds
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In contrast to transcripts, proteins, and metabolites, intracellular metabolic fluxes cannot be measured directly, but rather rely on prediction via computational simulation or on indirect measurements. In this chapter, we describe different approaches to quantify and simulate fluxes in plant metabolic pathways. Metabolic behavior can be simulated with models ranging from basic stoichiometry to fully detailed kinetic models, and fluxes can be quantified via a combination of computer modeling and stable isotope labeling experiments. We especially emphasize the usefulness of these approaches for improving the understanding of storage compound synthesis in developing crop seeds
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KRACH, Christian, Eva GRAFAHREND-BELAU, Hart POSKAR, Kai SCHALLAU, Falk SCHREIBER, Björn H. JUNKER, 2012. Using Systems Approaches to Analyze Metabolic Networks Involved in Storage Reserve Synthesis in Developing Seeds. In: AGRAWAL, Ganesh K., ed. and others. Seed development : OMICS technologies toward improvement of seed quality and crop yield; OMICS in seed biology. Dordrecht: Springer, 2012, pp. 387-405. ISBN 978-94-007-4748-7. Available under: doi: 10.1007/978-94-007-4749-4_19BibTex
@incollection{Krach2012-12-07Using-38511, year={2012}, doi={10.1007/978-94-007-4749-4_19}, title={Using Systems Approaches to Analyze Metabolic Networks Involved in Storage Reserve Synthesis in Developing Seeds}, isbn={978-94-007-4748-7}, publisher={Springer}, address={Dordrecht}, booktitle={Seed development : OMICS technologies toward improvement of seed quality and crop yield; OMICS in seed biology}, pages={387--405}, editor={Agrawal, Ganesh K.}, author={Krach, Christian and Grafahrend-Belau, Eva and Poskar, Hart and Schallau, Kai and Schreiber, Falk and Junker, Björn H.} }
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