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Quantitative visualization of endocytic trafficking through photoactivation of fluorescent proteins

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2021

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Ecker, Manuela
Redpath, Gregory M. I.
Nicovich, Philip R.

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Molecular biology of the cell. American Society for Cell Biology (ASCB). 2021, 32(9), pp. 892-902. ISSN 1044-2030. eISSN 1939-4586. Available under: doi: 10.1091/mbc.E20-10-0669

Zusammenfassung

Endocytic trafficking controls the density of molecules at the plasma membrane and by doing so, the cell surface profile, which in turn determines how cells interact with their environment. A full apprehension of any cellular process necessitates understanding how proteins associated with the plasma membrane are endocytosed, how they are sorted after internalization, and if and how they are recycled to the plasma membrane. To date, it is still difficult to experimentally gain access to this information, even more to do it in a quantitative way. Here we present a toolset based on photoactivation of fluorescent proteins that enabled us to generate quantitative information on endocytosis, incorporation into sorting and recycling endosomes, delivery from endosomes to the plasma membrane, and on the type of vesicles performing intracellular transport. We illustrate these approaches by revealing striking differences in the endocytic trafficking of T-cell receptor and CD4, which bind to the same molecule at the surface of antigen-presenting cells during T-cell activation.

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570 Biowissenschaften, Biologie

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ISO 690ECKER, Manuela, Gregory M. I. REDPATH, Philip R. NICOVICH, Jérémie ROSSY, 2021. Quantitative visualization of endocytic trafficking through photoactivation of fluorescent proteins. In: Molecular biology of the cell. American Society for Cell Biology (ASCB). 2021, 32(9), pp. 892-902. ISSN 1044-2030. eISSN 1939-4586. Available under: doi: 10.1091/mbc.E20-10-0669
BibTex
@article{Ecker2021Quant-53793,
  year={2021},
  doi={10.1091/mbc.E20-10-0669},
  title={Quantitative visualization of endocytic trafficking through photoactivation of fluorescent proteins},
  number={9},
  volume={32},
  issn={1044-2030},
  journal={Molecular biology of the cell},
  pages={892--902},
  author={Ecker, Manuela and Redpath, Gregory M. I. and Nicovich, Philip R. and Rossy, Jérémie}
}
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