Publikation:

Identification and Properties of New Flavins in Electron-Transferring Flavoprotein from Peptostreptococcus elsdenii and Pig-Liver Glycolate Oxidase

Vorschaubild

Dateien

Eur_J_Biochem_1974_MayhewIdentification_and_properties_of_new.pdf
Eur_J_Biochem_1974_MayhewIdentification_and_properties_of_new.pdfGröße: 2.22 MBDownloads: 363

Datum

1974

Autor:innen

Mayhew, Stephen G.
Whitfield, Carolyn D.
Schuman-Jörns, Marylin

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-55626
DOI (zitierfähiger Link)
https://doi.org/10.1111/j.1432-1033.1974.tb03515.x
ArXiv-ID

Internationale Patentnummer

Link zur Lizenz
Attribution-NonCommercial-NoDerivs 2.0 Generic

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green

Sammlungen

Biologie: Publikationen
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

European Journal of Biochemistry. 1974, 44(2), pp. 579-591. ISSN 0014-2956. eISSN 1432-1033. Available under: doi: 10.1111/j.1432-1033.1974.tb03515.x

Zusammenfassung

I. New flavins have been isolated from purified preparations of an electron-transferring flavoprotein (ETF) from Peptostreptococcus elsdenii and glycolate oxidase from pig liver. The structures of these new species have been established as FAD and FMN derivatives of 6-hydroxy-7,8-dimethyl-isoalloxazine, the chemical synthesis of which is described in the accompanying paper by Schöllnhammer and Hemmerich. The chromophores are yellow at pH 5 and green at pH 9 due to an ionization at pK 7.1.
2.6-OH-FAD is bound by apo-ETF and its pK is decreased. The complex is reduced by NADH and it couples the oxidation of NADH to the reduction of dichlorophenolindophenol. Unlike the complex of FAD and apo-ETF, 6-OH-FAD * ETF does not couple the oxidation of NADH to the reduction of butyryl-CoA dehydrogenase.
3.6-OH-FMN is bound by the FMN-specific protein apoflavodoxin from P, elsdenii and the pK is increased to ≈9. This complex is reduced by sodium dithionite and an intermediate, presumed to be the semi-quinone, is formed at half reduction.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690MAYHEW, Stephen G., Carolyn D. WHITFIELD, Sandro GHISLA, Marylin SCHUMAN-JÖRNS, 1974. Identification and Properties of New Flavins in Electron-Transferring Flavoprotein from Peptostreptococcus elsdenii and Pig-Liver Glycolate Oxidase. In: European Journal of Biochemistry. 1974, 44(2), pp. 579-591. ISSN 0014-2956. eISSN 1432-1033. Available under: doi: 10.1111/j.1432-1033.1974.tb03515.x
BibTex
@article{Mayhew1974Ident-7651,
  year={1974},
  doi={10.1111/j.1432-1033.1974.tb03515.x},
  title={Identification and Properties of New Flavins in Electron-Transferring Flavoprotein from Peptostreptococcus elsdenii and Pig-Liver Glycolate Oxidase},
  number={2},
  volume={44},
  issn={0014-2956},
  journal={European Journal of Biochemistry},
  pages={579--591},
  author={Mayhew, Stephen G. and Whitfield, Carolyn D. and Ghisla, Sandro and Schuman-Jörns, Marylin}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7651">
    <dc:contributor>Schuman-Jörns, Marylin</dc:contributor>
    <dcterms:abstract xml:lang="eng">I. New flavins have been isolated from purified preparations of an  electron-transferring flavoprotein (ETF) from Peptostreptococcus elsdenii and glycolate oxidase from pig liver. The structures of these new species have been established as FAD and FMN derivatives of 6-hydroxy-7,8-dimethyl-isoalloxazine, the chemical synthesis of which is described in the accompanying paper by Schöllnhammer and Hemmerich. The chromophores are yellow at pH 5 and green at pH 9 due to an ionization at pK 7.1.&lt;br /&gt;2.6-OH-FAD is bound by apo-ETF and its pK is decreased. The complex is reduced by NADH and it couples the oxidation of NADH to the reduction of dichlorophenolindophenol. Unlike the complex of FAD and apo-ETF, 6-OH-FAD * ETF does not couple the oxidation of NADH to the reduction of butyryl-CoA dehydrogenase.&lt;br /&gt;3.6-OH-FMN is bound by the FMN-specific protein apoflavodoxin from P, elsdenii and the pK is increased to ≈9. This complex is reduced by sodium dithionite and an intermediate, presumed to be the semi-quinone, is formed at half reduction.</dcterms:abstract>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7651/1/Eur_J_Biochem_1974_MayhewIdentification_and_properties_of_new.pdf"/>
    <dc:contributor>Mayhew, Stephen G.</dc:contributor>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:36:05Z</dcterms:available>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:36:05Z</dc:date>
    <dc:creator>Mayhew, Stephen G.</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dcterms:title>Identification and Properties of New Flavins in Electron-Transferring Flavoprotein from Peptostreptococcus elsdenii and Pig-Liver Glycolate Oxidase</dcterms:title>
    <dc:contributor>Ghisla, Sandro</dc:contributor>
    <dcterms:bibliographicCitation>First publ. in: European Journal of Biochemistry 44 (1974), 2, pp. 579-591</dcterms:bibliographicCitation>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7651"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Whitfield, Carolyn D.</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7651/1/Eur_J_Biochem_1974_MayhewIdentification_and_properties_of_new.pdf"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Schuman-Jörns, Marylin</dc:creator>
    <dc:creator>Whitfield, Carolyn D.</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:issued>1974</dcterms:issued>
    <dc:format>application/pdf</dc:format>
    <dc:creator>Ghisla, Sandro</dc:creator>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen