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Crystallization and preliminary X-ray crystallographic studies of the native and chemically modified anion-selective porin from Comamonas acidovorans

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1998

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Zeth, Kornelius
Schnaible, Volker
Engelhardt, Harald

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Acta Crystallographica Section D Biological Crystallography. 1998, 54(4), pp. 650-653. ISSN 0907-4449. eISSN 1399-0047. Available under: doi: 10.1107/S0907444997018854

Zusammenfassung

Omp32, the strongly anion-selective porin from Comamonas acidovorans, has been crystallized. Two crystal forms were observed, both of which belong to space group R3, but exhibit different cell dimensions a = b = 106.7, c = 140.6 A Ê (crystal form I) and a = b = 87.1, c = 135.3 A Ê (crystal form II) with one trimer per asymmetric unit. The crystals diffract to 2.2 and 2.3 A Ê resolution, respectively. Omp32 was chemically modified by introducing negative charges through succinylation. The number and positions of the individual modifications were determined using mass spectrometry and X-ray crystallography. Chemically modified porins yielded crystals of a third form, also of space group R3 but with cell constants of a = b = 109.3 and c = 263.2 A Ê (crystal form III), showing a virtually doubled c axis. Crystals of form III diffract to 3.5 A Ê resolution.

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570 Biowissenschaften, Biologie

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ISO 690ZETH, Kornelius, Volker SCHNAIBLE, Michael PRZYBYLSKI, Wolfram WELTE, Kay DIEDERICHS, Harald ENGELHARDT, 1998. Crystallization and preliminary X-ray crystallographic studies of the native and chemically modified anion-selective porin from Comamonas acidovorans. In: Acta Crystallographica Section D Biological Crystallography. 1998, 54(4), pp. 650-653. ISSN 0907-4449. eISSN 1399-0047. Available under: doi: 10.1107/S0907444997018854
BibTex
@article{Zeth1998Cryst-20913,
  year={1998},
  doi={10.1107/S0907444997018854},
  title={Crystallization and preliminary X-ray crystallographic studies of the native and chemically modified anion-selective porin from Comamonas acidovorans},
  number={4},
  volume={54},
  issn={0907-4449},
  journal={Acta Crystallographica Section D Biological Crystallography},
  pages={650--653},
  author={Zeth, Kornelius and Schnaible, Volker and Przybylski, Michael and Welte, Wolfram and Diederichs, Kay and Engelhardt, Harald}
}
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    <dcterms:abstract xml:lang="eng">Omp32, the strongly anion-selective porin from Comamonas acidovorans, has been crystallized. Two crystal forms were observed, both of which belong to space group R3, but exhibit different cell dimensions a = b = 106.7, c = 140.6 A Ê (crystal form I) and a = b = 87.1, c = 135.3 A Ê (crystal form II) with one trimer per asymmetric unit. The crystals diffract to 2.2 and 2.3 A Ê resolution, respectively. Omp32 was chemically modified by introducing negative charges through succinylation. The number and positions of the individual modifications were determined using mass spectrometry and X-ray crystallography. Chemically modified porins yielded crystals of a third form, also of space group R3 but with cell constants of a = b = 109.3 and c = 263.2 A Ê (crystal form III), showing a virtually doubled c axis. Crystals of form III diffract to 3.5 A Ê resolution.</dcterms:abstract>
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