Publikation:

Crystallization and preliminary X-ray crystallographic studies of the native and chemically modified anion-selective porin from Comamonas acidovorans

Vorschaubild

Dateien

4 se0220 edit.pdf
4 se0220 edit.pdfGröße: 1.95 MBDownloads: 249

Datum

1998

Autor:innen

Zeth, Kornelius
Schnaible, Volker
Engelhardt, Harald

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-209130
DOI (zitierfähiger Link)
https://doi.org/10.1107/S0907444997018854
ArXiv-ID

Internationale Patentnummer

Link zur Lizenz
Urheberrechtlich geschützt

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green

Sammlungen

Biologie: Publikationen
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Acta Crystallographica Section D Biological Crystallography. 1998, 54(4), pp. 650-653. ISSN 0907-4449. eISSN 1399-0047. Available under: doi: 10.1107/S0907444997018854

Zusammenfassung

Omp32, the strongly anion-selective porin from Comamonas acidovorans, has been crystallized. Two crystal forms were observed, both of which belong to space group R3, but exhibit different cell dimensions a = b = 106.7, c = 140.6 A Ê (crystal form I) and a = b = 87.1, c = 135.3 A Ê (crystal form II) with one trimer per asymmetric unit. The crystals diffract to 2.2 and 2.3 A Ê resolution, respectively. Omp32 was chemically modified by introducing negative charges through succinylation. The number and positions of the individual modifications were determined using mass spectrometry and X-ray crystallography. Chemically modified porins yielded crystals of a third form, also of space group R3 but with cell constants of a = b = 109.3 and c = 263.2 A Ê (crystal form III), showing a virtually doubled c axis. Crystals of form III diffract to 3.5 A Ê resolution.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690ZETH, Kornelius, Volker SCHNAIBLE, Michael PRZYBYLSKI, Wolfram WELTE, Kay DIEDERICHS, Harald ENGELHARDT, 1998. Crystallization and preliminary X-ray crystallographic studies of the native and chemically modified anion-selective porin from Comamonas acidovorans. In: Acta Crystallographica Section D Biological Crystallography. 1998, 54(4), pp. 650-653. ISSN 0907-4449. eISSN 1399-0047. Available under: doi: 10.1107/S0907444997018854
BibTex
@article{Zeth1998Cryst-20913,
  year={1998},
  doi={10.1107/S0907444997018854},
  title={Crystallization and preliminary X-ray crystallographic studies of the native and chemically modified anion-selective porin from Comamonas acidovorans},
  number={4},
  volume={54},
  issn={0907-4449},
  journal={Acta Crystallographica Section D Biological Crystallography},
  pages={650--653},
  author={Zeth, Kornelius and Schnaible, Volker and Przybylski, Michael and Welte, Wolfram and Diederichs, Kay and Engelhardt, Harald}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/20913">
    <dc:creator>Schnaible, Volker</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:title>Crystallization and preliminary X-ray crystallographic studies of the native and chemically modified anion-selective porin from Comamonas acidovorans</dcterms:title>
    <dc:creator>Welte, Wolfram</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-12-12T10:39:31Z</dcterms:available>
    <dc:creator>Diederichs, Kay</dc:creator>
    <dcterms:bibliographicCitation>Acta Crystallographica Section D : Biological Crystallography ; 54 (1998), 4. - S. 650-653</dcterms:bibliographicCitation>
    <dc:contributor>Engelhardt, Harald</dc:contributor>
    <dc:contributor>Schnaible, Volker</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/20913/2/4%20se0220%20edit.pdf"/>
    <dc:contributor>Diederichs, Kay</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/20913"/>
    <dc:language>eng</dc:language>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:rights>terms-of-use</dc:rights>
    <dc:contributor>Welte, Wolfram</dc:contributor>
    <dc:creator>Zeth, Kornelius</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Zeth, Kornelius</dc:contributor>
    <dc:contributor>Przybylski, Michael</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-12-12T10:39:31Z</dc:date>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/20913/2/4%20se0220%20edit.pdf"/>
    <dc:creator>Przybylski, Michael</dc:creator>
    <dc:creator>Engelhardt, Harald</dc:creator>
    <dcterms:abstract xml:lang="eng">Omp32, the strongly anion-selective porin from Comamonas acidovorans, has been crystallized. Two crystal forms were observed, both of which belong to space group R3, but exhibit different cell dimensions a = b = 106.7, c = 140.6 A Ê (crystal form I) and a = b = 87.1, c = 135.3 A Ê (crystal form II) with one trimer per asymmetric unit. The crystals diffract to 2.2 and 2.3 A Ê resolution, respectively. Omp32 was chemically modified by introducing negative charges through succinylation. The number and positions of the individual modifications were determined using mass spectrometry and X-ray crystallography. Chemically modified porins yielded crystals of a third form, also of space group R3 but with cell constants of a = b = 109.3 and c = 263.2 A Ê (crystal form III), showing a virtually doubled c axis. Crystals of form III diffract to 3.5 A Ê resolution.</dcterms:abstract>
    <dcterms:issued>1998</dcterms:issued>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen