Publikation:

Diamondoid-modified DNA

Vorschaubild

Dateien

Marx.pdf
Marx.pdfGröße: 653.91 KBDownloads: 271

Datum

2011

Autor:innen

Tkachenko, Boryslav A.
Schreiner, Peter R.

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-163591
DOI (zitierfähiger Link)
https://doi.org/10.1039/c1ob05929g
ArXiv-ID

Internationale Patentnummer

Link zur Lizenz
Urheberrechtlich geschützt

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green

Sammlungen

Chemie: Publikationen
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Organic & Biomolecular Chemistry. 2011, 9(21), pp. 7482-7490. ISSN 1477-0520. eISSN 1477-0539. Available under: doi: 10.1039/c1ob05929g

Zusammenfassung

We prepared novel C5-modified triphosphates and phosphoramidites with a diamondoid functionally linked to the nucleobase. Using primer extension experiments with different length templates we investigated whether the modified triphosphates were enzymatically incorporated into DNA and whether they were further extended. We found that all three modified nucleotides can be incorporated into DNA using a single-nucleotide incorporation experiment, but only partially using two templates that demand for multiple incorporation of the modified nucleotides. The modified phosphoramidites were introduced into oligonucleotides utilizing DNA synthesizer technology. The occurring oligonucleotide structures were examined by circular dichroism (CD) and melting temperature (Tm)measurements and were found to adapt similar helix conformations as their unmodified counterparts.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
540 Chemie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690WANG, Yan, Boryslav A. TKACHENKO, Peter R. SCHREINER, Andreas MARX, 2011. Diamondoid-modified DNA. In: Organic & Biomolecular Chemistry. 2011, 9(21), pp. 7482-7490. ISSN 1477-0520. eISSN 1477-0539. Available under: doi: 10.1039/c1ob05929g
BibTex
@article{Wang2011-11-07Diamo-16359,
  year={2011},
  doi={10.1039/c1ob05929g},
  title={Diamondoid-modified DNA},
  number={21},
  volume={9},
  issn={1477-0520},
  journal={Organic & Biomolecular Chemistry},
  pages={7482--7490},
  author={Wang, Yan and Tkachenko, Boryslav A. and Schreiner, Peter R. and Marx, Andreas}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/16359">
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/16359/2/Marx.pdf"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Schreiner, Peter R.</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/16359/2/Marx.pdf"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Marx, Andreas</dc:creator>
    <dc:creator>Wang, Yan</dc:creator>
    <dc:contributor>Tkachenko, Boryslav A.</dc:contributor>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:rights>terms-of-use</dc:rights>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-26T11:00:15Z</dc:date>
    <dcterms:abstract xml:lang="eng">We prepared novel C5-modified triphosphates and phosphoramidites with a diamondoid functionally linked to the nucleobase. Using primer extension experiments with different length templates we investigated whether the modified triphosphates were enzymatically incorporated into DNA and whether they were further extended. We found that all three modified nucleotides can be incorporated into DNA using a single-nucleotide incorporation experiment, but only partially using two templates that demand for multiple incorporation of the modified nucleotides. The modified phosphoramidites were introduced into oligonucleotides utilizing DNA synthesizer technology. The occurring oligonucleotide structures were examined by circular dichroism (CD) and melting temperature (Tm)measurements and were found to adapt similar helix conformations as their unmodified counterparts.</dcterms:abstract>
    <dc:contributor>Wang, Yan</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-08-31T22:25:04Z</dcterms:available>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/16359"/>
    <dc:contributor>Schreiner, Peter R.</dc:contributor>
    <dc:creator>Tkachenko, Boryslav A.</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:issued>2011-11-07</dcterms:issued>
    <dcterms:bibliographicCitation>First publ. in: Organic &amp; Biomolecular Chemistry ; 9 (2011), 21. - S. 7482-7490</dcterms:bibliographicCitation>
    <dc:contributor>Marx, Andreas</dc:contributor>
    <dcterms:title>Diamondoid-modified DNA</dcterms:title>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen