Publikation: Association of spin-labeled lipids with beta-barrel proteins from the outer membrane of Escherichia coli
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The interaction of spin-labeled lipids with β-barrel transmembrane proteins has been studied by the electron spin resonance (ESR) methods developed for α-helical integral proteins. The outer membrane protein OmpA and the ferrichrome-iron receptor FhuA from the outer membrane of Escherichia coli were reconstituted in bilayers of dimyristoylphosphatidylglycerol. The ESR spectra from phosphatidylglycerol spin labeled on the 14-C atom of the sn-2 chain contain a second component from motionally restricted lipids contacting the intramembranous surface of the β-barrel, in addition to that from the fluid bilayer lipids. The stoichiometry of motionally restricted lipids, 11 and 32 lipids/monomer for OmpA and FhuA, respectively, is constant irrespective of the total lipid/protein ratio. It is proportional to the number of transmembrane β-strands, eight for OmpA and 22 for FhuA, and correlates reasonably well with the intramembranous perimeter of the protein. Spin-labeled lipids with different polar headgroups display a differential selectivity of interaction with the two proteins. The more pronounced pattern of lipid selectivity for FhuA than for OmpA correlates with the preponderance of positively charged residues facing the lipids in the extensions of the β-sheet and shorter interconnecting loops on the extracellular side of FhuA.
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RAMAKRISHNAN, Muthu, Cosmin L. POCANSCHI, Jörg KLEINSCHMIDT, Derek MARSH, 2004. Association of spin-labeled lipids with beta-barrel proteins from the outer membrane of Escherichia coli. In: Biochemistry. 2004, 43(37), pp. 11630-11636. ISSN 0006-2960. eISSN 1520-4995BibTex
@article{Ramakrishnan2004Assoc-7367, year={2004}, title={Association of spin-labeled lipids with beta-barrel proteins from the outer membrane of Escherichia coli}, number={37}, volume={43}, issn={0006-2960}, journal={Biochemistry}, pages={11630--11636}, author={Ramakrishnan, Muthu and Pocanschi, Cosmin L. and Kleinschmidt, Jörg and Marsh, Derek} }
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