Mass spectrometric approaches for elucidation of antigen antibody recognition structures in molecular immunology

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EJMS_2007_Stefanescu_.pdf
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2007
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Stefanescu, Raluca
Iacob, Roxana Elena
Damoc, Eugen
Amstalden, Erika
Perdivara, Irina
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European Journal of Mass Spectrometry. 2007, 13(1), pp. 69-75. ISSN 1356-1049. Available under: doi: 10.1255/ejms.849
Zusammenfassung

Mass spectrometric approaches have recently gained increasing access to molecular immunology and several methods have been developed that enable detailed chemical structure identification of antigen-antibody interactions. Selective proteolytic digestion and MS-peptide mapping (epitope excision) has been successfully employed for epitope identification of protein antigens. In addition, affinity proteomics using partial epitope excision has been developed as an approach with unprecedented selectivity for direct protein identification from biological material. The potential of these methods is illustrated by the elucidation of a β- amyloid plaque-specific epitope recognized by therapeutic antibodies from transgenic mouse models of Alzheimer s disease. Using an immobilized antigen and antibody- proteolytic digestion and analysis by high resolution Fourier transform ion cyclotron resonance mass spectrometry has lead to a new approach for the identification of antibody paratope structures (paratope-excision; parexprot ). In this method, high resolution MS-peptide data at the low ppm level are required for direct identification of paratopes using protein databases. Mass spectrometric epitope mapping and determination of molecular antibody-recognition signatures offer high potential, especially for the development of new molecular diagnostics and the evaluation of new vaccine lead structures.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
affinity mass spectrometry, proteolytic epitope excision, antibody paratope, Alzheimer´s Disease, FTICR-MS
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ISO 690STEFANESCU, Raluca, Roxana Elena IACOB, Eugen DAMOC, Andreas MARQUARDT, Erika AMSTALDEN, Marilena MANEA, Irina PERDIVARA, Madalina MAFTEI, Gabriela-Ioana PARASCHIV, Michael PRZYBYLSKI, 2007. Mass spectrometric approaches for elucidation of antigen antibody recognition structures in molecular immunology. In: European Journal of Mass Spectrometry. 2007, 13(1), pp. 69-75. ISSN 1356-1049. Available under: doi: 10.1255/ejms.849
BibTex
@article{Stefanescu2007spect-9969,
  year={2007},
  doi={10.1255/ejms.849},
  title={Mass spectrometric approaches for elucidation of antigen antibody recognition structures in molecular immunology},
  number={1},
  volume={13},
  issn={1356-1049},
  journal={European Journal of Mass Spectrometry},
  pages={69--75},
  author={Stefanescu, Raluca and Iacob, Roxana Elena and Damoc, Eugen and Marquardt, Andreas and Amstalden, Erika and Manea, Marilena and Perdivara, Irina and Maftei, Madalina and Paraschiv, Gabriela-Ioana and Przybylski, Michael}
}
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