Publikation:

From morphology to biochemical state : intravital multiphoton fluorescence lifetime imaging of inflamed human skin

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Huck_0-327696.pdf
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2016

Autor:innen

Huck, Volker
Gorzelanny, Christian
Thomas, Kai
Getova, Valentina
Niemeyer, Verena
Zens, Katharina
Unnerstall, Tim R.
Feger, Julia S.
Schneider, Stefan W.
et al.

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http://nbn-resolving.de/urn:nbn:de:bsz:352-0-327696
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https://doi.org/10.1038/srep22789
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CC BY 4.0

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Open Access-Veröffentlichung
Open Access Gold

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Chemie: Publikationen
Core Facility der Universität Konstanz

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Scientific Reports. 2016, 6, 22789. eISSN 2045-2322. Available under: doi: 10.1038/srep22789

Zusammenfassung

The application of multiphoton microscopy in the field of biomedical research and advanced diagnostics promises unique insights into the pathophysiology of inflammatory skin diseases. In the present study, we combined multiphoton-based intravital tomography (MPT) and fluorescence lifetime imaging (MPT-FLIM) within the scope of a clinical trial of atopic dermatitis with the aim of providing personalised data on the aetiopathology of inflammation in a non-invasive manner at patients' bedsides. These 'optical biopsies' generated via MPT were morphologically analysed and aligned with classical skin histology. Because of its subcellular resolution, MPT provided evidence of a redistribution of mitochondria in keratinocytes, indicating an altered cellular metabolism. Two independent morphometric algorithms reliably showed an even distribution in healthy skin and a perinuclear accumulation in inflamed skin. Moreover, using MPT-FLIM, detection of the onset and progression of inflammatory processes could be achieved. In conclusion, the change in the distribution of mitochondria upon inflammation and the verification of an altered cellular metabolism facilitate a better understanding of inflammatory skin diseases and may permit early diagnosis and therapy.

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540 Chemie

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ISO 690HUCK, Volker, Christian GORZELANNY, Kai THOMAS, Valentina GETOVA, Verena NIEMEYER, Katharina ZENS, Tim R. UNNERSTALL, Julia S. FEGER, Mohammad A. FALLAH, Stefan W. SCHNEIDER, 2016. From morphology to biochemical state : intravital multiphoton fluorescence lifetime imaging of inflamed human skin. In: Scientific Reports. 2016, 6, 22789. eISSN 2045-2322. Available under: doi: 10.1038/srep22789
BibTex
@article{Huck2016morph-34013,
  year={2016},
  doi={10.1038/srep22789},
  title={From morphology to biochemical state : intravital multiphoton fluorescence lifetime imaging of inflamed human skin},
  volume={6},
  journal={Scientific Reports},
  author={Huck, Volker and Gorzelanny, Christian and Thomas, Kai and Getova, Valentina and Niemeyer, Verena and Zens, Katharina and Unnerstall, Tim R. and Feger, Julia S. and Fallah, Mohammad A. and Schneider, Stefan W.},
  note={Article Number: 22789}
}
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