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The subunits MECL-1 and LMP2 are mutually required for incorporation into the 20S proteasome

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1997

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Standera, Sybille
Stohwasser, Ralf
Kloetzel, Peter M.

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" Proceedings of the National Academy of Sciences of the United States of America : PNAS. 1997, 94(17), pp. 8970-8975. ISSN 0027-8424. eISSN 1091-6490

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Processing of antigens for presentation by major histocompatibility complex (MHC) class I molecules requires the activity of the proteasome. The 20S proteasome complex is composed of 14 different subunits, 2 of which can be substituted by the interferon gamma (IFN-gamma)-inducible and MHC-encoded subunits LMP2 and LMP7 (low molecular mass poylpeptides 2 and 7). A third subunit, MECL-1, is inducible by IFN-gamma but is encoded outside the MHC. Here we show by cotransfection experiments that the incorporation of MECL-1 into the 20S proteasome is directly dependent on the expression of LMP2 but independent of LMP7. Conversely, the uptake of LMP2 is strongly enhanced by MECL-1 expression. The expression of MECL-1 caused a replacement of the homologous subunit Z in the 20S proteasome complex. LMP2 is required for MECL-1 incorporation at the level of proteasome precursor formation that guarantees the concerted incorporation of two IFN-gamma-inducible proteasome subunits encoded inside and outside the MHC. The obligatory coincorporation of MECL-1 and LMP2 is an important parameter for the interpretation of results obtained with LMP2-deficient cell lines and mice as well as for the design of experiments addressing the function of MECL-1 in antigen presentation.

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570 Biowissenschaften, Biologie

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ISO 690GRÖTTRUP, Marcus, Sybille STANDERA, Ralf STOHWASSER, Peter M. KLOETZEL, 1997. The subunits MECL-1 and LMP2 are mutually required for incorporation into the 20S proteasome. In: " Proceedings of the National Academy of Sciences of the United States of America : PNAS. 1997, 94(17), pp. 8970-8975. ISSN 0027-8424. eISSN 1091-6490
BibTex
@article{Grottrup1997subun-22243,
  year={1997},
  title={The subunits MECL-1 and LMP2 are mutually required for incorporation into the 20S proteasome},
  number={17},
  volume={94},
  issn={0027-8424},
  journal={" Proceedings of the National Academy of Sciences of the United States of America : PNAS},
  pages={8970--8975},
  author={Gröttrup, Marcus and Standera, Sybille and Stohwasser, Ralf and Kloetzel, Peter M.}
}
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