Publikation:

Achieving Single-Nucleotide Resolution of 5-Methylcytosine Detection with TALEs

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2015

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https://doi.org/10.1002/cbic.201402408
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Chemie: Publikationen
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ChemBioChem. 2015, 16(2), pp. 228-231. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201402408

Zusammenfassung

We report engineered transcription-activator-like effectors (TALEs) as the first DNA-binding molecules that detect 5-methylcytosine (mC) at single-nucleotide resolution with fully programmable sequence selectivity. This is achieved by a design strategy such that a single cytosine (C) in a DNA sequence is selectively interrogated for its mC-modification level by targeting with a discriminatory TALE repeat; other Cs are ignored by targeting with universal-binding TALE repeats.

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540 Chemie

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DNA methylation, epigenetics, programmable protein scaffolds, sensors, TALE proteins

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ISO 690KUBIK, Grzegorz, Daniel SUMMERER, 2015. Achieving Single-Nucleotide Resolution of 5-Methylcytosine Detection with TALEs. In: ChemBioChem. 2015, 16(2), pp. 228-231. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201402408
BibTex
@article{Kubik2015Achie-30723,
  year={2015},
  doi={10.1002/cbic.201402408},
  title={Achieving Single-Nucleotide Resolution of 5-Methylcytosine Detection with TALEs},
  number={2},
  volume={16},
  issn={1439-4227},
  journal={ChemBioChem},
  pages={228--231},
  author={Kubik, Grzegorz and Summerer, Daniel}
}
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