Publikation: Nanog-like Regulates Endoderm Formation through the Mxtx2-Nodal Pathway
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2012
Autor:innen
Xu, Cong
Fan, Zi Peng
Fogley, Rachel
DiBiase, Anthony
Trompouki, Eirini
Unternaehrer, Juli
Xiong, Fengzhu
Torregroza, Ingrid
Zon, Leonard I.
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Developmental Cell. Cell Press. 2012, 22(3), pp. 625-638. ISSN 1534-5807. eISSN 1878-1551. Available under: doi: 10.1016/j.devcel.2012.01.003
Zusammenfassung
In mammalian embryonic stem cells, the acquisition of pluripotency is dependent on Nanog, but the in vivo analysis of Nanog has been hampered by its requirement for early mouse development. In an effort to examine the role of Nanog in vivo, we identified a zebrafish Nanog ortholog and found that its knockdown impaired endoderm formation. Genome-wide transcription analysis revealed that nanog-like morphants fail to develop the extraembryonic yolk syncytial layer (YSL), which produces Nodal, required for endoderm induction. We examined the genes that were regulated by Nanog-like and identified the homeobox gene mxtx2, which is both necessary and sufficient for YSL induction. Chromatin immunoprecipitation assays and genetic studies indicated that Nanog-like directly activates mxtx2, which, in turn, specifies the YSL lineage by directly activating YSL genes. Our study identifies a Nanog-like-Mxtx2-Nodal pathway and establishes a role for Nanog-like in regulating the formation of the extraembryonic tissue required for endoderm induction.
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570 Biowissenschaften, Biologie
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XU, Cong, Zi Peng FAN, Patrick MÜLLER, Rachel FOGLEY, Anthony DIBIASE, Eirini TROMPOUKI, Juli UNTERNAEHRER, Fengzhu XIONG, Ingrid TORREGROZA, Leonard I. ZON, 2012. Nanog-like Regulates Endoderm Formation through the Mxtx2-Nodal Pathway. In: Developmental Cell. Cell Press. 2012, 22(3), pp. 625-638. ISSN 1534-5807. eISSN 1878-1551. Available under: doi: 10.1016/j.devcel.2012.01.003BibTex
@article{Xu2012-03-13Nanog-56287,
year={2012},
doi={10.1016/j.devcel.2012.01.003},
title={Nanog-like Regulates Endoderm Formation through the Mxtx2-Nodal Pathway},
number={3},
volume={22},
issn={1534-5807},
journal={Developmental Cell},
pages={625--638},
author={Xu, Cong and Fan, Zi Peng and Müller, Patrick and Fogley, Rachel and DiBiase, Anthony and Trompouki, Eirini and Unternaehrer, Juli and Xiong, Fengzhu and Torregroza, Ingrid and Zon, Leonard I.}
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<dcterms:abstract xml:lang="eng">In mammalian embryonic stem cells, the acquisition of pluripotency is dependent on Nanog, but the in vivo analysis of Nanog has been hampered by its requirement for early mouse development. In an effort to examine the role of Nanog in vivo, we identified a zebrafish Nanog ortholog and found that its knockdown impaired endoderm formation. Genome-wide transcription analysis revealed that nanog-like morphants fail to develop the extraembryonic yolk syncytial layer (YSL), which produces Nodal, required for endoderm induction. We examined the genes that were regulated by Nanog-like and identified the homeobox gene mxtx2, which is both necessary and sufficient for YSL induction. Chromatin immunoprecipitation assays and genetic studies indicated that Nanog-like directly activates mxtx2, which, in turn, specifies the YSL lineage by directly activating YSL genes. Our study identifies a Nanog-like-Mxtx2-Nodal pathway and establishes a role for Nanog-like in regulating the formation of the extraembryonic tissue required for endoderm induction.</dcterms:abstract>
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