Cre-inducible site-specific recombination in zebrafish oligodendrocytes

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2017
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Chekuru, Avinash
Bosak, Viktoria
Brand, Michael
Hans, Stefan
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Developmental Dynamics. 2017, 246(1), pp. 41-49. ISSN 1058-8388. eISSN 1553-0795. Available under: doi: 10.1002/dvdy.24458
Zusammenfassung

Background: The conditional Cre/lox system has recently emerged as a valuable tool for studies on both embryonic and adult Zebrafish. Temporal control and site-specific recombination are achieved by using the ligand-inducible CreERT2 and administration of the drug tamoxifen (TAM) or its active metabolite, 4-Hydroxytamoxifen (4-OHT).
Results: Here we report the generation of a transgenic Zebrafish line, which expresses an mCherry-tagged variant of CreERT2 under the control of the myelin basic protein a (mbpa) promoter. Our analysis shows that larval and adult expression of the transgene recapitulates the endogenous mbpa expression pattern in oligodendrocytes. Furthermore, combination with a Cre-dependent EGFP reporter results in EGFP-expressing oligodendrocytes in the spinal cord, brain, and optic nerve in TAM- or 4-OHT-treated larvae and 4-month-old fish, but not in untreated controls.
Conclusions: The transgenic Zebrafish line Tg(mbpa:mCherry-T2A-CreERT2) elicits CreERT2 expression specifically in myelinating glia cells. Cre-inducible targeted recombination of genes in oligodendrocytes will be useful to elucidate cellular and molecular mechanisms of myelination in vivo during development (myelination) and regeneration (remyelination) after injury to the central nervous system (CNS). It will also allow targeted expression and overexpression of genes of interest (transgenes) in oligodendrocytes at defined developmental and adult stages.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie
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oligodendrocytes; Cre/lox system; myelin basic protein promoter; glia cell-specific recombination; transgenic Zebrafish; myelination
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ISO 690PINZON-OLEJUA, Alejandro, Cornelia WELTE, Avinash CHEKURU, Viktoria BOSAK, Michael BRAND, Stefan HANS, Claudia STÜRMER, 2017. Cre-inducible site-specific recombination in zebrafish oligodendrocytes. In: Developmental Dynamics. 2017, 246(1), pp. 41-49. ISSN 1058-8388. eISSN 1553-0795. Available under: doi: 10.1002/dvdy.24458
BibTex
@article{PinzonOlejua2017-01Crein-37311,
  year={2017},
  doi={10.1002/dvdy.24458},
  title={Cre-inducible site-specific recombination in zebrafish oligodendrocytes},
  number={1},
  volume={246},
  issn={1058-8388},
  journal={Developmental Dynamics},
  pages={41--49},
  author={Pinzon-Olejua, Alejandro and Welte, Cornelia and Chekuru, Avinash and Bosak, Viktoria and Brand, Michael and Hans, Stefan and Stürmer, Claudia}
}
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