Publikation:

One-way calcium spill-over during signal transduction in Parameciumcells : from the cell cortex into cilia, but not in the reverse direction

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Datum

2004

Autor:innen

Husser, Marc R.
Hardt, Martin
Blanchard, Marie-Pierre
Klauke, Norbert

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http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-42286
DOI (zitierfähiger Link)
https://doi.org/10.1016/j.ceca.2004.02.003
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Attribution-NonCommercial-NoDerivs 2.0 Generic

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Open Access-Veröffentlichung
Open Access Green

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Biologie: Publikationen
Core Facility der Universität Konstanz

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Published

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Cell Calcium. 2004, 36(5), pp. 349-358. ISSN 0143-4160. Available under: doi: 10.1016/j.ceca.2004.02.003

Zusammenfassung

We asked to what extent Ca2+ signals in two different domains of Paramecium cells remain separated during different stimulations. Wild-type (7S) and pawn cells (strain d4-500r, without ciliary voltage-dependent Ca2+-channels) were stimulated for trichocyst exocytosis within 80 ms by quenched-flow preparation and analysed by energy-dispersive X-ray microanalysis (EDX), paralleled by fast confocal fluorochrome analysis. We also analysed depolarisation-dependent calcium signalling during ciliary beat rerversal, also by EDX, after 80-ms stimulation in the quenched-flow mode. EDX and fluorochrome analysis enable to register total and free intracellular calcium concentrations, [Ca] and [Ca2+], respectively. After exocytosis stimulation we find by both methods that the calcium signal sweeps into the basis of cilia, not only in 7S but also in pawn cells which then also perform ciliary reversal. After depolarisation we see an increase of [Ca] along cilia selectively in 7S, but not in pawn cells. Opposite to exocytosis stimulation, during depolarisation no calcium spill-over into the nearby cytosol and no exocytosis occurs. In sum, we conclude that cilia must contain a very potent Ca2+ buffering system and that ciliary reversal induction, much more than exocytosis stimulation, involves strict microdomain regulation of Ca2+ signals.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Ca2+, Calcium, Cilia, EDX, Paramecium, X-ray microanalysis

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ISO 690HUSSER, Marc R., Martin HARDT, Marie-Pierre BLANCHARD, Joachim HENTSCHEL, Norbert KLAUKE, Helmut PLATTNER, 2004. One-way calcium spill-over during signal transduction in Parameciumcells : from the cell cortex into cilia, but not in the reverse direction. In: Cell Calcium. 2004, 36(5), pp. 349-358. ISSN 0143-4160. Available under: doi: 10.1016/j.ceca.2004.02.003
BibTex
@article{Husser2004Onewa-7701,
  year={2004},
  doi={10.1016/j.ceca.2004.02.003},
  title={One-way calcium spill-over during signal transduction in Parameciumcells : from the cell cortex into cilia, but not in the reverse direction},
  number={5},
  volume={36},
  issn={0143-4160},
  journal={Cell Calcium},
  pages={349--358},
  author={Husser, Marc R. and Hardt, Martin and Blanchard, Marie-Pierre and Hentschel, Joachim and Klauke, Norbert and Plattner, Helmut}
}
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