Publikation:

A Genetically Encoded Spin Label for Electron Paramagnetic Resonance Distance Measurements

Vorschaubild

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2014

Autor:innen

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-278086
DOI (zitierfähiger Link)
https://doi.org/10.1021/ja411535q
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung

Sammlungen

Chemie: Publikationen
Zukunftskolleg: Publikationen
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Journal of the American Chemical Society. 2014, 136(4), pp. 1238-1241. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/ja411535q

Zusammenfassung

We report the genetic encoding of a noncanonical, spin-labeled amino acid in Escherichia coli. This enables the intracellular biosynthesis of spin-labeled proteins and obviates the need for any chemical labeling step usually required for protein electron paramagnetic resonance (EPR) studies. The amino acid can be introduced at multiple, user-defined sites of a protein and is stable in E. coli even for prolonged expression times. It can report intramolecular distance distributions in proteins by double-electron electron resonance measurements. Moreover, the signal of spin-labeled protein can be selectively detected in cells. This provides elegant new perspectives for in-cell EPR studies of endogenous proteins.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
540 Chemie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690SCHMIDT, Moritz J., Julia BORBAS, Malte DRESCHER, Daniel SUMMERER, 2014. A Genetically Encoded Spin Label for Electron Paramagnetic Resonance Distance Measurements. In: Journal of the American Chemical Society. 2014, 136(4), pp. 1238-1241. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/ja411535q
BibTex
@article{Schmidt2014-01-29Genet-27808,
  year={2014},
  doi={10.1021/ja411535q},
  title={A Genetically Encoded Spin Label for Electron Paramagnetic Resonance Distance Measurements},
  number={4},
  volume={136},
  issn={0002-7863},
  journal={Journal of the American Chemical Society},
  pages={1238--1241},
  author={Schmidt, Moritz J. and Borbas, Julia and Drescher, Malte and Summerer, Daniel}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/27808">
    <dc:creator>Drescher, Malte</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dcterms:issued>2014-01-29</dcterms:issued>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:bibliographicCitation>Journal of the American Chemical Society ; 136 (2014), 4. - S. 1238–1241</dcterms:bibliographicCitation>
    <dc:creator>Borbas, Julia</dc:creator>
    <dc:contributor>Summerer, Daniel</dc:contributor>
    <dc:contributor>Borbas, Julia</dc:contributor>
    <dc:contributor>Drescher, Malte</dc:contributor>
    <dc:language>eng</dc:language>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:abstract xml:lang="eng">We report the genetic encoding of a noncanonical, spin-labeled amino acid in Escherichia coli. This enables the intracellular biosynthesis of spin-labeled proteins and obviates the need for any chemical labeling step usually required for protein electron paramagnetic resonance (EPR) studies. The amino acid can be introduced at multiple, user-defined sites of a protein and is stable in E. coli even for prolonged expression times. It can report intramolecular distance distributions in proteins by double-electron electron resonance measurements. Moreover, the signal of spin-labeled protein can be selectively detected in cells. This provides elegant new perspectives for in-cell EPR studies of endogenous proteins.</dcterms:abstract>
    <dc:creator>Summerer, Daniel</dc:creator>
    <dc:contributor>Schmidt, Moritz J.</dc:contributor>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:title>A Genetically Encoded Spin Label for Electron Paramagnetic Resonance Distance Measurements</dcterms:title>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2014-05-13T14:18:52Z</dcterms:available>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2014-05-13T14:18:52Z</dc:date>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/27808"/>
    <dc:creator>Schmidt, Moritz J.</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen