A Genetically Encoded Spin Label for Electron Paramagnetic Resonance Distance Measurements

Schmidt, Moritz J.; Borbas, Julia; Drescher, Malte; Summerer, Daniel

A Genetically Encoded Spin Label for Electron Paramagnetic Resonance Distance Measurements

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Datum

2014

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Journal of the American Chemical Society. 2014, 136(4), pp. 1238-1241. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/ja411535q

Zusammenfassung

We report the genetic encoding of a noncanonical, spin-labeled amino acid in Escherichia coli. This enables the intracellular biosynthesis of spin-labeled proteins and obviates the need for any chemical labeling step usually required for protein electron paramagnetic resonance (EPR) studies. The amino acid can be introduced at multiple, user-defined sites of a protein and is stable in E. coli even for prolonged expression times. It can report intramolecular distance distributions in proteins by double-electron electron resonance measurements. Moreover, the signal of spin-labeled protein can be selectively detected in cells. This provides elegant new perspectives for in-cell EPR studies of endogenous proteins.

Fachgebiet (DDC)

540 Chemie

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ISO 690

SCHMIDT, Moritz J., Julia BORBAS, Malte DRESCHER, Daniel SUMMERER, 2014. A Genetically Encoded Spin Label for Electron Paramagnetic Resonance Distance Measurements. In: Journal of the American Chemical Society. 2014, 136(4), pp. 1238-1241. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/ja411535q

BibTex

@article{Schmidt2014-01-29Genet-27808,
  year={2014},
  doi={10.1021/ja411535q},
  title={A Genetically Encoded Spin Label for Electron Paramagnetic Resonance Distance Measurements},
  number={4},
  volume={136},
  issn={0002-7863},
  journal={Journal of the American Chemical Society},
  pages={1238--1241},
  author={Schmidt, Moritz J. and Borbas, Julia and Drescher, Malte and Summerer, Daniel}
}

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