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Thermostable protein-stabilized gold nanoclusters as a peroxidase mimic

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2023

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Nanoscale Advances. Royal Society of Chemistry (RSC). 2023, 5(22), S. 6061-6068. eISSN 2516-0230. Verfügbar unter: doi: 10.1039/d3na00566f

Zusammenfassung

Protein-stabilized gold nanoclusters (AuNCs) are fascinating nanostructures with exciting properties owing to their ultra-small sizes and functional shell. However, their applications under extreme conditions are still complicated, waiting for programmable solutions. Therefore, the design of a multi-functional protein stabilizer for specific purposes gains attention to improve the stability and functionality of AuNCs. Herein, we exploited the thermostability of genetically engineered KlenTaq DNA polymerase containing five cysteine residues (KTQ5C) to synthesize heat-stable AuNCs (AuNC@KTQ5C) and characterize optical, structural, and hydrodynamic properties. Besides their excellent photophysical properties, AuNC@KTQ5C also exhibit superior peroxidase-like (POD-like) catalytic activity following typical Michaelis–Menten kinetics together with a high affinity towards the POD substrate 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt (ABTS). Moreover, FTIR and relative catalytic activity analysis of AuNC@KTQ5C reveal that KTQ5C is resistant to changes in protein secondary structure while the AuNCs conserve 70–80% of their catalytic performance after heat treatments up to more than 80 °C. Our findings show that stabilizing AuNCs with thermostable KTQ5C not only preserves the advantages of protein-stabilized AuNCs but can also promote the resistance of AuNCs against aggregation due to protein denaturation under extreme reaction temperatures, protecting their fluorescent emission or catalytic activity.

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570 Biowissenschaften, Biologie

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General Engineering

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ISO 690AKYÜZ, Özlem, Maite MISSUN, Rose ROSENBERG, Martin SCHEFFNER, Andreas MARX, Helmut CÖLFEN, 2023. Thermostable protein-stabilized gold nanoclusters as a peroxidase mimic. In: Nanoscale Advances. Royal Society of Chemistry (RSC). 2023, 5(22), S. 6061-6068. eISSN 2516-0230. Verfügbar unter: doi: 10.1039/d3na00566f
BibTex
@article{Akyuz2023Therm-67908,
  year={2023},
  doi={10.1039/d3na00566f},
  title={Thermostable protein-stabilized gold nanoclusters as a peroxidase mimic},
  number={22},
  volume={5},
  journal={Nanoscale Advances},
  pages={6061--6068},
  author={Akyüz, Özlem and Mißun, Maite and Rosenberg, Rose and Scheffner, Martin and Marx, Andreas and Cölfen, Helmut}
}
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