Publikation:

X-ray analysis of protein crystals with thin-plate morphology

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Mayans_2-ssk9tidbx8302.pdf
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Datum

1999

Autor:innen

Wilmanns, Matthias

Herausgeber:innen

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URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-2-ssk9tidbx8302
DOI (zitierfähiger Link)
https://doi.org/10.1107/S0909049599004616
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Open Access-Veröffentlichung
Open Access Green

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Biologie: Publikationen
Core Facility der Universität Konstanz

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Published

Erschienen in

Journal of Synchrotron Radiation. Wiley. 1999, 6, pp. 1016-1020. ISSN 0909-0495. eISSN 1600-5775. Available under: doi: 10.1107/S0909049599004616

Zusammenfassung

A case study on quasi two-dimensional crystals of the serine kinase domain of the giant muscle protein titin is presented. These crystals were obtained from several precipitating agents. However, under all conditions their growth in the third dimension was inhibited resulting in very thin plates, hardly visible under the microscope. Here a number of aspects that have been essential for high-resolution data collection from these crystals are described: (i) improvement of the crystals by fine tuning their growth, namely by the addition of organic solvents, the application of macroseeding, and the reduction of vapour diffusion in the crystal drop vials using oil mixtures over the reservoir solutions; (ii) cryomounting techniques preventing mechanical stress; and (iii) optimizing the use of synchrotron radiation.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

protein crystals; plate morphology; titin kinase

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ISO 690MAYANS, Olga, Matthias WILMANNS, 1999. X-ray analysis of protein crystals with thin-plate morphology. In: Journal of Synchrotron Radiation. Wiley. 1999, 6, pp. 1016-1020. ISSN 0909-0495. eISSN 1600-5775. Available under: doi: 10.1107/S0909049599004616
BibTex
@article{Mayans1999analy-52480,
  year={1999},
  doi={10.1107/S0909049599004616},
  title={X-ray analysis of protein crystals with thin-plate morphology},
  volume={6},
  issn={0909-0495},
  journal={Journal of Synchrotron Radiation},
  pages={1016--1020},
  author={Mayans, Olga and Wilmanns, Matthias}
}
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