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Cation-Pi Interactions as Determinants for Binding of the Compatible Solutes Glycine Betaine and Proline Betaine by the Periplasmic Ligand-binding Protein ProX from Escherichia coli

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2004

Autor:innen

Schiefner, André
Breed, Jason
Bösser, Linda
Kneip, Susanne
Gade, Jutta
Holtmann, Gudrun
Bremer, Erhard

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Published

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The Journal of Biological Chemistry. 2004, 279(7), pp. 5588-5596. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M309771200

Zusammenfassung

Compatible solutes such as glycine betaine and proline betaine are accumulated to exceedingly high intracellular levels by many organisms in response to high osmolarity to offset the loss of cell water. They are excluded from the immediate hydration shell of proteins and thereby stabilize their native structure. Despite their exclusion from protein surfaces, the periplasmic ligand-binding protein ProX from the Escherichia coli ATP-binding cassette transport system ProU binds the compatible solutes glycine betaine and proline betaine with high affinity and specificity. To understand the mechanism of compatible solute binding, we determined the high resolution structure of ProX in complex with its ligands glycine betaine and proline betaine. This crystallographic study revealed that cation-π interactions between the positive charge of the quaternary amine of the ligands and three tryptophan residues forming a rectangular aromatic box are the key determinants of the high affinity binding of compatible solutes by ProX. The structural analysis was combined with site-directed mutagenesis of the ligand binding pocket to estimate the contributions of the tryptophan residues involved in binding.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

GB, glycine betaine (N,N,N-trimethylglycine), PB, proline betaine (N,N-dimethyl-L-proline), X-gal

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ISO 690SCHIEFNER, André, Jason BREED, Linda BÖSSER, Susanne KNEIP, Jutta GADE, Gudrun HOLTMANN, Kay DIEDERICHS, Wolfram WELTE, Erhard BREMER, 2004. Cation-Pi Interactions as Determinants for Binding of the Compatible Solutes Glycine Betaine and Proline Betaine by the Periplasmic Ligand-binding Protein ProX from Escherichia coli. In: The Journal of Biological Chemistry. 2004, 279(7), pp. 5588-5596. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M309771200
BibTex
@article{Schiefner2004Catio-7786,
  year={2004},
  doi={10.1074/jbc.M309771200},
  title={Cation-Pi Interactions as Determinants for Binding of the Compatible Solutes Glycine Betaine and Proline Betaine by the Periplasmic Ligand-binding Protein ProX from Escherichia coli},
  number={7},
  volume={279},
  issn={0021-9258},
  journal={The Journal of Biological Chemistry},
  pages={5588--5596},
  author={Schiefner, André and Breed, Jason and Bösser, Linda and Kneip, Susanne and Gade, Jutta and Holtmann, Gudrun and Diederichs, Kay and Welte, Wolfram and Bremer, Erhard}
}
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