Increased Levels of Antigen-Bound beta-Amyloid Autoantibodies in Serum and Cerebrospinal Fluid of Alzheimer's Disease Patients

Recent studies have suggested a protective role of physiological β-amyloid autoantibodies (Aβ-autoantibodies) in Alzheimer's disease (AD). However, the determination of both free and dissociated Aβ-autoantibodies in serum hitherto has yielded inconsistent results regarding their function and possible biomarker value. Here we report the application of a new sandwich enzyme-linked immunosorbent assay (ELISA) for the determination of antigen-bound Aβ-autoantibodies (intact Aβ-IgG immune complexes) in serum and cerebrospinal fluid (CSF) of a total number of 112 AD patients and age- and gender-matched control subjects. Both serum and CSF levels of Aβ-IgG immune complexes were found to be significantly higher in AD patients compared to control subjects. Moreover, the levels of Aβ-IgG complexes were negatively correlated with the cognitive status across the groups, increasing with declining cognitive test performance of the subjects. Our results suggest a contribution of IgG-type autoantibodies to Aβ clearance in vivo and an increased immune response in AD, which may be associated with deficient Aβ-IgG removal. These findings may contribute to elucidating the role of Aβ-autoantibodies in AD pathophysiology and their potential application in AD diagnosis.

Fachgebiet (DDC)

540 Chemie

Zitieren

ISO 690

MAFTEI, Madalina, Franka THURM, Cathrin SCHNACK, Hayrettin TUMANI, Markus OTTO, Thomas ELBERT, Iris-Tatjana KOLASSA, Michael PRZYBYLSKI, Marilena MANEA, Christine A. F. von ARNIM, 2013. Increased Levels of Antigen-Bound beta-Amyloid Autoantibodies in Serum and Cerebrospinal Fluid of Alzheimer's Disease Patients. In: PLoS ONE. 2013, 8(7), e68996. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0068996

BibTex

@article{Maftei2013Incre-24148,
  year={2013},
  doi={10.1371/journal.pone.0068996},
  title={Increased Levels of Antigen-Bound beta-Amyloid Autoantibodies in Serum and Cerebrospinal Fluid of Alzheimer's Disease Patients},
  number={7},
  volume={8},
  journal={PLoS ONE},
  author={Maftei, Madalina and Thurm, Franka and Schnack, Cathrin and Tumani, Hayrettin and Otto, Markus and Elbert, Thomas and Kolassa, Iris-Tatjana and Przybylski, Michael and Manea, Marilena and Arnim, Christine A. F. von},
  note={Article Number: e68996}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/24148">
    <dc:creator>Tumani, Hayrettin</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/24148"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Elbert, Thomas</dc:creator>
    <dc:contributor>Thurm, Franka</dc:contributor>
    <dc:contributor>Elbert, Thomas</dc:contributor>
    <dc:contributor>Manea, Marilena</dc:contributor>
    <dc:creator>Maftei, Madalina</dc:creator>
    <dc:contributor>Arnim, Christine A. F. von</dc:contributor>
    <dc:contributor>Maftei, Madalina</dc:contributor>
    <dc:contributor>Przybylski, Michael</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-07-29T06:58:11Z</dc:date>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dc:creator>Przybylski, Michael</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dc:creator>Schnack, Cathrin</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/24148/1/Maftei_241481.pdf"/>
    <dcterms:title>Increased Levels of Antigen-Bound beta-Amyloid Autoantibodies in Serum and Cerebrospinal Fluid of Alzheimer's Disease Patients</dcterms:title>
    <dc:creator>Otto, Markus</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/24148/1/Maftei_241481.pdf"/>
    <dc:contributor>Otto, Markus</dc:contributor>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Schnack, Cathrin</dc:contributor>
    <dcterms:abstract xml:lang="eng">Recent studies have suggested a protective role of physiological β-amyloid autoantibodies (Aβ-autoantibodies) in Alzheimer's disease (AD). However, the determination of both free and dissociated Aβ-autoantibodies in serum hitherto has yielded inconsistent results regarding their function and possible biomarker value. Here we report the application of a new sandwich enzyme-linked immunosorbent assay (ELISA) for the determination of antigen-bound Aβ-autoantibodies (intact Aβ-IgG immune complexes) in serum and cerebrospinal fluid (CSF) of a total number of 112 AD patients and age- and gender-matched control subjects. Both serum and CSF levels of Aβ-IgG immune complexes were found to be significantly higher in AD patients compared to control subjects. Moreover, the levels of Aβ-IgG complexes were negatively correlated with the cognitive status across the groups, increasing with declining cognitive test performance of the subjects. Our results suggest a contribution of IgG-type autoantibodies to Aβ clearance in vivo and an increased immune response in AD, which may be associated with deficient Aβ-IgG removal. These findings may contribute to elucidating the role of Aβ-autoantibodies in AD pathophysiology and their potential application in AD diagnosis.</dcterms:abstract>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-07-29T06:58:11Z</dcterms:available>
    <dcterms:issued>2013</dcterms:issued>
    <dc:contributor>Kolassa, Iris-Tatjana</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>Manea, Marilena</dc:creator>
    <dc:contributor>Tumani, Hayrettin</dc:contributor>
    <dcterms:bibliographicCitation>PLoS ONE ; 8 (2013), 7. - e68996</dcterms:bibliographicCitation>
    <dc:language>eng</dc:language>
    <dc:creator>Arnim, Christine A. F. von</dc:creator>
    <dc:creator>Thurm, Franka</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Kolassa, Iris-Tatjana</dc:creator>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja