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Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity

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2022

Autor:innen

Zafar, Sana
Makitrynskyy, Roman
Bechthold, Andreas
Song, Lijiang
Anwar, Munir Ahmad
Luzhetskyy, Andriy
Khan, Ali Nisar
Khaliq, Shazia
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Antibiotics. MDPI Publishing. 2022, 11(8), 1057. eISSN 2079-6382. Available under: doi: 10.3390/antibiotics11081057

Zusammenfassung

Actinomycetes, most notably the genus Streptomyces, have great importance due to their role in the discovery of new natural products, especially for finding antimicrobial secondary metabolites that are useful in the medicinal science and biotechnology industries. In the current study, a genome-based evaluation of Streptomyces sp. isolate BR123 was analyzed to determine its biosynthetic potential, based on its in vitro antimicrobial activity against a broad range of microbial pathogens, including gram-positive and gram-negative bacteria and fungi. A draft genome sequence of 8.15 Mb of Streptomyces sp. isolate BR123 was attained, containing a GC content of 72.63% and 8103 protein coding genes. Many antimicrobial, antiparasitic, and anticancerous compounds were detected by the presence of multiple biosynthetic gene clusters, which was predicted by in silico analysis. A novel metabolite with a molecular mass of 1271.7773 in positive ion mode was detected through a high-performance liquid chromatography linked with mass spectrometry (HPLC-MS) analysis. In addition, another compound, meridamycin, was also identified through a HPLC-MS analysis. The current study reveals the biosynthetic potential of Streptomyces sp. isolate BR123, with respect to the synthesis of bioactive secondary metabolites through genomic and spectrometric analysis. Moreover, the comparative genome study compared the isolate BR123 with other Streptomyces strains, which may expand the knowledge concerning the mechanism involved in novel antimicrobial metabolite synthesis.

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570 Biowissenschaften, Biologie

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Streptomyces; secondary metabolites; genome; biosynthetic gene clusters; high-performance liquid chromatography (HPLC); mass spectrometry

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ISO 690ASHRAF, Neelma, Sana ZAFAR, Roman MAKITRYNSKYY, Andreas BECHTHOLD, Dieter SPITELLER, Lijiang SONG, Munir Ahmad ANWAR, Andriy LUZHETSKYY, Ali Nisar KHAN, Shazia KHALIQ, 2022. Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity. In: Antibiotics. MDPI Publishing. 2022, 11(8), 1057. eISSN 2079-6382. Available under: doi: 10.3390/antibiotics11081057
BibTex
@article{Ashraf2022-08-04Revea-58246,
  year={2022},
  doi={10.3390/antibiotics11081057},
  title={Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity},
  number={8},
  volume={11},
  journal={Antibiotics},
  author={Ashraf, Neelma and Zafar, Sana and Makitrynskyy, Roman and Bechthold, Andreas and Spiteller, Dieter and Song, Lijiang and Anwar, Munir Ahmad and Luzhetskyy, Andriy and Khan, Ali Nisar and Khaliq, Shazia},
  note={Article Number: 1057}
}
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